Rat mAb: A-647-anti-mouse VCAM-1 (CD106, clone 429)

Company: BioLegend

Catalog#: 105712

Using the Sleeping Beauty Transposon System for Doxycycline-inducible Gene Expression in RAW264.7 Macrophage Cells to Study Phagocytosis

Parsa Kamali and Gregory D. Fairn

Published online: 2025-02-05

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Co-immunostaining of ICAM-1, ICAM-2, and CD31 in Mouse Kidney Glomeruli

Author:

Sun-sang J. Sung,

Date:

2020-07-05

[Abstract] Glomerulonephritis (GN) is a common pathological condition in chronic kidney diseases that often leads to end stage renal failure. Mac-1 (CD11b/CD18)-mediated neutrophil, macrophage, and dendritic cell glomerular infiltration leading to cellular dysfunction and destruction is an important disease mechanism. The cellular distribution and dynamics of the expression of Mac-1 ligands ICAM-1 and ICAM-2 in GN have not been well studied because of the difficulties in tissue staining and colocalizing glomerular cells with surface antigens. To improve the visualization of cell surface marker and antigen expression in kidney compartments, we have devised an even but mild fixation procedure employing p-formaldehyde-lysine-periodate (PLP) perfusion. A large panel of antibodies (Ab) against cell ... [摘要] [摘要 ] 肾小球肾炎（GN）是慢性肾脏疾病的常见病理状况，通常会导致晚期肾衰竭。Mac-1（CD11b / CD18）介导的嗜中性粒细胞，巨噬细胞和树突状细胞肾小球浸润导致细胞功能障碍和破坏是重要的疾病机制。由于在组织染色和肾小球细胞与表面抗原共定位方面存在困难，因此尚未对GN中Mac-1配体ICAM-1和ICAM-2的表达进行细胞分布和动力学研究。为了改善肾脏区室中细胞表面标志物和抗原表达的可视化，我们设计了一种均匀而温和的固定方法，即使用对甲醛-高赖氨酸-高碘酸盐（PLP）灌注。一大批针对细胞表面标志物的抗体（Ab）用于鉴定肾细胞类型和粘附分子。当使用共聚焦显微镜观察肾小球粘附分子染色时，发现内皮细胞特异性表达CD31，并且这些细胞组成性表达ICAM-2。尽管ICAM-1在稳态中不由肾小球内皮细胞表达，但在患有慢性GN和严重蛋白尿的小鼠中ICAM-1高度上调。VCAM-1是在白细胞迁移中重要的VLA-4配体，未在肾小球中表达。结果强调了ICAM-1在巨噬细胞和树突状细胞浸润中的重要性。cGN 。该报告将为产生高质量共聚焦图像以及鉴定和定量在不同肾区室和细胞类型中表达的受体和其他细胞抗原提供广泛适用的程序。

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Rat mAb: A-647-anti-mouse VCAM-1 (CD106, clone 429)

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