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Author:
Date:
2020-06-20
[Abstract] The interaction between the host plant Arabidopsis thaliana (Arabidopsis) and the oomycete Hyaloperonospora arabidopsidis (Hpa) is an established model system for the study of an obligate biotrophic downy mildew interaction. The evaluation of the developmental success of Hpa is often based on the quantification of reproductive structures that are formed on the surface of leaves, such as the sporangiophores or the conidiospores they carry. However, the structural basis of this interaction lies within the plant tissue and, in particular, the haustoria that form inside plant cells. Therefore, valuable additional information about the performance and compatibility of the downy mildew interaction can be gained by light microscopical inspection of the hyphal ...
[摘要] [摘要] 寄主植物拟南芥（Arabidopsis thaliana，Arabidopsis）与卵菌的相互作用是研究专性生物营养性霜霉病相互作用的模型系统。对Hpa发育成功的评价通常是基于对叶片表面形成的生殖结构的量化，如孢子囊或其携带的分生孢子。然而，这种相互作用的结构基础在于植物组织，特别是在植物细胞内形成的吸器。因此，通过对植物组织内和细胞内菌丝形态和吸器形态的光镜观察，可以获得有关霜霉病相互作用的性能和相容性的有价值的附加信息。这里我们描述了一个可视化和量化植物内部形态表型的协议。虽然我们特别关注吸器形状变量的量化，但该协议可以很容易地适用于其他形态学特征的量化，如菌丝变形或卵原细胞频率。通过对已有的各种来源的协议进行归纳和提炼，我们组装了拟南芥Hpa生物测定的整个实验管道，为该系统在实验室的初步建立提供了实用的指导。该管道包括以下步骤：A）生长拟南芥；B）Hpa繁殖和菌株保持；C）Hpa接种和培养；D）植物组织染色以显示病原体；E）引入Keyence VHX显微镜和ImageJ的Fiji插件以量化感兴趣的结构。虽然这里描述的是拟南芥和Hpa，但B-E方案步骤应该很容易调整，以研究其他植物卵菌的病理系统。活体营养型卵菌（百帕）
[背景] ...
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