Labscreen Single Antigen HLA class-I Combination bead suspension array, which contains 97 common HLA-A, -B, and -C bound beads derived from individual cell lines, a positive control bead sample, and a negative control bead sample, where each bead is distinguished based on the fluorescence intensity readout

Company: One Lambda Inc.

Catalog#: Class-I-LS1A04NC

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Assessments of HLA-I Specificities of Anti-HLA-I Monoclonal Antibodies Using Solid Phase Bead Arrays

Author:

Anita J. Zaitouna, Daniel S. Ramon and Malini Raghavan,

Date:

2020-06-20

[Abstract] Human leukocyte antigen class I (HLA-I) molecules are a group of structurally-related cell surface proteins with a high degree of variability within the population. While only up to six variants are expressed in an individual person, the whole population contains thousands of different variants. The ability to distinguish specific variants is important in the clinic to determine compatibility during organ and bone marrow transplantation and in the laboratory to study the biological properties of individual variants. Solid phase bead arrays contain purified, individually identifiable HLA-I molecules that can be used to determine antibody specificity for individual HLA-I proteins. This method is high-throughput, highly specific, and allows for simultaneous screening of antibodies against ... [摘要] [摘要 ] 人类白细胞抗原I类（HLA-1）分子是一组与结构相关的细胞表面蛋白，在群体中具有高度可变性。一个人最多只能表达六个变体，但整个群体中包含数千个在临床中确定器官和骨髓移植的相容性以及在实验室研究单个变体的生物学特性时，区分特定变体的能力很重要。固相微珠阵列包含纯化的，可单独识别的HLA-1分子可以用于确定单个HLA-1蛋白的抗体特异性。该方法具有高通量，高特异性，可以同时筛选针对多种HLA-1同种异型的抗体。磁珠特别适用于筛查患者血清中的存在针对单个HLA-1变体的供体特异性抗体（可能在怀孕期间出现，b 输血或器官移植）等替代方法（例如使用单个表达HLA-1的细胞系）更加耗时，并且此类细胞系难以采购和标准化。研究HLA-1对其他受体和结合伴侣的特异性和选择性。

[背景 ] 人类白细胞抗原分子分为I类（HLA-I）或II类（HLA-II）。一般而言，HLA-1在所有有核细胞上均被发现并与CD8 + T细胞的T细胞受体结合，从而形成HLA -II在称为抗原呈递细胞的专门免疫细胞上发现，并与CD4 + T细胞的T细胞受体结合。HLA-I和HLA-II蛋白的主要受体是T细胞受体和CD8或CD4协同受体虽然其它受体，蛋白质和抗体是已知的，以在功能上与两组molecules.HLA-I类分子相互作用包括高度多态性的重链，一个不变的轻链（β ...

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