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Luciferin
Company: Wako
Catalog#: 126-05116
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Ex vivo Culture Assay Using Human Hair Follicles to Study Circadian Characteristics
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2020-06-05
[Abstract]  Ex vivo culture assays of biopsy specimens are advantageous for the experimental evaluation of human circadian characteristics. We developed a simple and non-invasive experimental evaluation method for monitoring the expression of circadian clock genes in an ex vivo culture assay using human hair follicles. This method imposes little burden on subjects. This assay is useful for validating correlations between circadian characteristics in hair follicles and intrinsic characteristics observed in physiological and behavioral studies. While they should be further validated, this ex vivo method constitutes a useful tool for estimating in vivo circadian characteristics. [摘要]  [摘要] 活检标本的体外培养测定法有利于人体昼夜节律特征的实验评估。我们开发了一种简单而无创的实验评估方法，用于监测人发体外培养法中昼夜节律基因的表达follicles.This方法强加subjects.This测定法是用于验证昼夜CH之间的相关性有用的小负担在毛囊和固有特性在生理和行为studies.While观察aracteristics它们应该被进一步验证，该离体方法用于小的有用工具体内估计 昼夜节律特征。

[背景] 活的生物体表现出在生理和行为的昼夜节律由生物钟驱动（杨和Kay，2001）。该昼夜发条由转录的细胞自主性和时钟基因驱动的负反馈环路（邓拉普，1999 ）。在哺乳动物中，转录因子BMAL1和CLOCK 通过E-box元件激活时钟和与时钟相关的基因（例如Period （Per ）和Cryptochrome （Cry ））的转录.PER与有效的转录抑制剂CRY一起起作用负性调节这一复杂（里珀特织女，2002年）。在体内评估个体的内在节律特点在人类，要么以恒定的例行或强制去同步化协议，价格昂贵，耗力。因此，评估利用离体培养试验中要估计体内的昼夜节律特征可能具有重要的优势。例如，一些研究已经结束，n个外周细胞反映了个体的昼夜节律偏好，称为计时型（Brown 等人，2005； Hida 等人，2013 ...

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