|
Author:
Date:
2020-06-05
[Abstract] Parasites of the genus Leishmania infect the mammalian hosts, including mice and humans and cause cutaneous or visceral leishmaniasis depending upon the parasite species transmitted by the vector sandfly. Leishmania amazonensis is one of the Leishmania species responsible for the cutaneous form of the disease. We have inoculated with these parasites the ear dermis of mice. RNA preparations were performed from fragmented tissues using a buffer containing guanidin isothiocynate (RLT buffer, RNeasy Mini Kit, Qiagen, SAS, France) and β-mercaptoethanol. Both reagents facilitate the isolation of intact RNA from tissues and the use of the RNeasy Kits present with several advantages that facilitate the isolation of pure non-degraded total RNA: i) This method allows to ...
[摘要] [摘要] 寄生虫 利什曼原虫感染哺乳动物宿主，包括小鼠和人类的原因皮肤或内脏Leishm Aniasis根据在转交了该载体的寄生虫种类白蛉。利什曼原虫亚马孙是的利什曼原虫物种负责对皮肤型疾病。我们已经接种这些RNA制备物从零散组织用含有缓冲液中进行guanidin 异硫氰酸（RLT缓冲液，RNeasy Mini试剂盒，Qiagen公司，SAS，法国）和β - 巯基乙醇。 两种试剂促进完整的RNA的从组织中分离，并与促进纯非恶化的总RNA的分离几个优点使用RNeasy试剂试剂盒本的：我）Ť 他的方法允许避免苯酚的在RNA提取存在缓冲液，通常在备选方案中使用;ⅱ）此外焦碳酸二乙酯（DEPC）处理的玻璃器皿，以避免RNA酶的样品的污染，不与该协议所需的;ⅲ）˚F inally，它是一个快速的过程和分离的总RNA可将其浓缩在小体积中，以利于其用于下游实验程序。
[背景] 属的病原体利什曼出生动物传染病与多种临床表现和疾病严重程度可变性的原因矢量（曼索托等人，2007）利什曼病的皮肤.The形式是由几个引起利什曼原虫种包括利什曼原虫亚马孙（麦克马洪-Pratt and Alexander，2004; Christensen et al。，2019）。
小鼠模型的存在，对皮肤利什曼病的敏感性高，中度或无敏感性，促进了寄生虫传播实验规程的发展（de ...
|