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Cell strainers
Company: Fisherbrand
Catalog#: 22363548
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Pancreatic Acinar Cell Preparation for Oxygen Consumption and Lactate Production Analysis
Author:
Date:
2020-05-20
[Abstract]  Mitochondrial dysfunction is a principal feature of acute pancreatitis (AP) although the underlying mechanisms are still unclear. AP precipitants induce Ca2+-dependent formation of the mitochondrial permeability transition pore (MPTP) in pancreatic acinar cells (PACs), leading to ATP depletion and necrosis. Evaluations of mitochondrial bioenergetics have mainly been performed in isolated PACs using confocal microscopy, with assessment of mitochondrial membrane potential, NADH/FAD+ and ATP levels, coupled with patch-clamp electrophysiology. These studies are technically demanding and time-consuming. Application of Seahorse flux analysis now allows detailed investigations of bioenergetics changes to be performed in cell populations using a multi-well plate-reader ... [摘要]  [摘要 ] 线粒体功能障碍是急性胰腺炎（AP）的主要特征，尽管其潜在机制尚不清楚。AP沉淀剂诱导胰腺腺泡细胞（PACs）中线粒体通透性过渡孔（MPTP）的Ca 2+ 依赖性形成，导致ATP耗竭和坏死。线粒体生物能学的评估主要使用共聚焦显微镜在分离的PAC中进行，评估线粒体膜电位，NADH / FAD + 和ATP水平，以及膜片钳电生理学。这些研究在技术上既费时又费力。应用范围 海马通量分析现在允许使用多孔平板阅读器格式对细胞群体中的生物能学变化进行详细研究；耗氧率（OCR）和细胞外酸化率（ECAR）分别提供有关细胞呼吸和糖酵解的重要信息。诸如最大呼吸，ATP关联容量和质子泄漏等参数可以通过应用呼吸功能“压力”测试得出，该测试涉及电子传输链的药理处理。因此，使用Seahorse Flux分析提供了一种快速，方便的方法来测量详细的细胞生物能学，并使结果与其他基于平板读取器的测定法结合使用，从而更全面地了解线粒体生物能学改变的病理生理后果。

[背景 ] 线粒体功能障碍是急性胰腺炎（AP）的核心特征，急性胰腺炎是一种使人衰弱且可能致命的疾病，目前尚无针对性的治疗方法（Criddle，2016； Habtezion ...

Flow Cytometry Analysis and Fluorescence-activated Cell Sorting of Myeloid Cells from Lung and Bronchoalveolar Lavage Samples from Mycobacterium tuberculosis-infected Mice
Author:
Date:
2020-05-20
[Abstract]  Mycobacterium tuberculosis (Mtb) is transmitted by aerosol and can cause serious bacterial infection in the lung that can be fatal if left untreated. Mtb is now the leading cause of death worldwide by an infectious agent. Characterizing the early events of in vivo infection following aerosol challenge is critical for understanding how innate immune cells respond to infection but is technically challenging due to the small number of bacteria that initially infect the lung. Previous studies either evaluated Mtb-infected cells at later stages of infection when the number of bacteria in the lung is much higher or used in vitro model systems to assess the response of myeloid cells to Mtb. Here, we describe a method that uses fluorescent bacteria, a high-dose aerosol ... [摘要]  [摘要 ] 结核分枝杆菌（Mtb）通过气溶胶传播，可引起严重的肺部细菌感染，如果不及时治疗，可能致命。Mtb现在已成为全球传染病致死的主要原因。表征气溶胶激发后体内感染的早期事件对于了解先天免疫细胞如何对感染做出反应至关重要，但由于最初会感染肺的细菌数量少，因此在技术上具有挑战性。先前的研究或者在肺部细菌数量高得多时在感染后期评估Mtb感染的细胞，或者在体外使用 评估骨髓细胞对Mtb反应的模型系统。在这里，我们介绍一种使用荧光细菌，大剂量气溶胶感染模型和流式细胞术跟踪气溶胶感染和荧光激活细胞分选（FACS）之后立即分离肺中Mtb感染细胞的方法，以分离幼稚的旁观者，和Mtb感染的细胞用于下游应用，包括RNA测序。该协议提供了在肺环境中监视Mtb感染和细胞特异性反应的能力，已知该环境可调节常驻和募集人群的功能。使用此协议，我们发现肺泡巨噬细胞通过上调受转录因子Nrf2调节并有害于细菌早期控制的细胞保护性转录反应，在体内对Mtb感染作出反应。

[背景 ] 气溶胶传播是结核分枝杆菌（Mtb）感染自然周期的关键组成部分，有助于细菌的毒性并导致其在肺部的独特感染模式（North ，1995；Riley 等，1995）。 ; Pai et ...

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