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Quantification of Bacterial Loads in Caenorhabditis elegans

Alyssa C. Walker, Rohan Bhargava, Alfonso S. Vaziriyan-Sani, Amanda S. Brust and Daniel M. Czyz

Published online: 2022-01-20

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Bacterial Lawn Avoidance and Bacterial Two Choice Preference Assays in Caenorhabditis elegans

Author:

Jogender Singh and Alejandro Aballay,

Date:

2020-05-20

[Abstract] Physical avoidance of pathogens is a crucial defense strategy used by the host to reduce pathogen infection. Hosts display the use of multiple strategies to sense and avoid pathogens, ranging from olfaction to sensing of damage caused by pathogen infection. Understanding various mechanisms of pathogen avoidance has the potential to uncover conserved host defense responses that are important against pathogen infections. Here, we describe protocols for studying pathogen lawn avoidance behavior as well as a change of bacterial preferences in the model nematode Caenorhabditis elegans. Besides, we describe the protocol for measuring preferences for pathogenic and nonpathogenic bacteria after training of the animals on pathogenic bacteria. These assays can be implemented in ... [摘要] [摘要 ] 物理避免病原体是宿主减少病原体感染的关键防御策略。主持人展示了使用多种策略来感知和避免病原体，从嗅觉到感知由病原体感染引起的损害。了解避免病原体的各种机制有可能揭示保守的宿主防御反应，这些反应对病原体感染很重要。在这里，我们描述协议研究线虫秀丽隐杆线虫模型中的病原体避免草坪行为以及细菌偏好的变化的协议。此外，我们描述了在病原菌上对动物进行训练后测量病原性和非病原性细菌偏好的协议。这些测定法可用于发现宿主学习的各种机制，从而避免病原体。

[背景 ] 一种宿主使用多个策略来抵御病原体感染（Medzhitov 等人，2012）。物理规避病原体是宿主使用的各种防御策略之一（Medzhitov 等，2012；Kavaliers 等，2019； Singh和Aballay ，2020）。在检测到细菌毒素后，不同的感觉机制（包括化学感受和伤害感受神经元引起的疼痛诱发）会导致回避行为。对导致病原体回避的机制的更深入了解，有可能揭示保守的宿主防御反应，这些反应对病原体感染很重要。

线虫秀丽隐杆线虫已被广泛用于理解病原体回避行为和相关学习。秀丽隐杆线虫似乎使用多种机制来了解导致逃避行为的诱因的病原体（Singh和Aballay ，2020年）。秀丽隐杆线虫可以感知细菌代谢产物（Tran 等，2017）以及核心细胞活动的扰动（Melo ...

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