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Author:
Date:
2020-12-20
[Abstract] The mRNA therapeutics is a new class of medicine to treat many various diseases. However, in vitro transcribed (IVT) mRNA triggers immune responses due to recognition by human endosomal and cytoplasmic RNA sensors, but incorporation of modified nucleosides have been shown to reduce such responses. Therefore, an assay signifying important aspects of the human immune system is still required. Here, we present a simple ex vivo method called ‘RNA ImmunoGenic Assay’ to measure immunogenicity of IVT-mRNAs in human whole blood. Chemically modified and unmodified mRNA are complexed with a transfection reagent (TransIT), and co-incubated in human whole blood. Specific cytokines are measured (TNF-α, INF-α, INF-γ, IL-6 and IL-12p70) using ELISAs. The ...
[摘要] [摘要] mRNA疗法是治疗多种疾病的新型药物。然而，我Ñ体外转录（IVT）的mRNA触发由于人类内体和细胞质RNA传感器，但是修饰的核苷的掺入识别的免疫应答已经示出吨ö减少此类反应。牛逼herefore ，测定标志着重要的环节人体免疫系统仍然需要。这里，我们提出一个简单的离体称为“RNA方法免疫ģ ENIC测定”测量的IVT-mRNA的免疫原性小号在人全血。将化学修饰和未修饰的mRNA与转染试剂（TransIT ）复合，并在人全血中共同孵育。特异性细胞因子测定（TNF- α ，INF- α ，INF- γ ，IL-6和IL-12p70的），使用的ELISA。进行qPCR分析以揭示特异性免疫途径的激活。所述RNA免疫ģ ENIC测定提供小号的简单且快速的方法来检测供体特异性-针对mRNA的治疗剂的免疫应答。
图形摘要：
RNA免疫基因测定的示意图
[背景] mRNA治疗是基因治疗的重要一类（Sahin等，2014 ；Antony等，2015 ...
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