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Author:
Date:
2020-04-20
[Abstract] Cell migration is a fundamental cellular process that plays a crucial role in many physioglogical and pathological processes such as wound healing or cancer metastasis. Many assays have been developed to examine cell migration, such as the wound healing or scratch assay, Boyden Chamber or transwell assay, and the method we will describe here, single cell migration assay. In this assay, cells are plated sparsely on a collagen coated plate and live cell imaging is performed over a period of 2 h at 1 frame per minute. After imaging is completed, cells are tracked manually using ImageJ by tracking movement of the centroid of the cell. These data points are then exported and overall distance travelled from frame to frame is determined and divided by total time imaged to determine speed of the ...
[摘要] [摘要 ] 细胞迁移是一个基本的细胞过程，在许多生理和病理过程中（如伤口愈合或癌症转移）起着至关重要的作用，已经开发了许多检测细胞迁移的方法，例如伤口愈合或刮擦实验，Boyden Chamber或Transwell 分析，我们将在此介绍的方法是单细胞迁移分析。在此分析中，将细胞稀疏地铺在胶原蛋白包被的板上，并在2小时内进行活细胞成像 成像完成后，通过跟踪细胞质心的运动使用ImageJ手动跟踪细胞，然后导出这些数据点，确定帧与帧之间的总距离并除以成像的总时间确定细胞的速度。此方法提供了一种在进行更复杂的测定之前检查细胞操作对细胞迁移影响的快速方法。
[背景 ] 细胞迁移在从胚胎发育到血管生成和肿瘤转移的生理和病理过程中均起着重要作用（Le Clainche 和Carlier ，2008）。细胞运动是高度精心策划的事件，可以概括为四个基本步骤的循环：我）膜伸出驱动下肌动蛋白聚合，二）稳定突出通过整合素介导的细胞-基质粘附，III）细胞体易位驱动通过肌动球蛋白收缩力，最后，IV）后发布结果的Mechan 的iCal力和/或细胞基质粘附组分的蛋白水解作用（Sheetz 等，1999 ; Ridley 等，2003; Panetti 等，2004; Stradal and Scita ，2006; Tomasevic 等，2007; Le Clainche and ...
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