Plasminogen

Company: Sigma-Aldrich

Catalog#: P7999-5UN

Fractionation of Native Protein Complexes from Mammalian Cells to Determine the Differential Proteasome Activity and Abundance

Ana Paula Zen Petisco Fiore and Christine Vogel

Published online: 2023-09-20

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Fibrin Breakdown Assay

Author:

Jiayue Ling, Connor M. Blair and George S. Baillie,

Date:

2020-04-20

[Abstract] Fibrinolysis is an integral part of the matrix remodeling process that contributes to tissue repair. Fibrin clots are broken down during fibrinolysis in a controlled process. Fibrin degradation products (FDPs) have also been shown to have a role in the regulation of cell growth and are implicated in various vascular diseases. This protocol was designed to quantitatively measure the extent of fibrin breakdown and how this can be adapted as a tool to further investigate the pathway involved in fibrinolysis or fibrin degradation products. Until now, we haven’t found an alternative method to analysis fibrinolysis. [摘要] [摘要] 纤维蛋白溶解是基质重塑过程中不可或缺的一部分，它有助于组织修复。在受控的过程中，纤维蛋白凝块在纤维蛋白溶解过程中被破坏。纤维蛋白降解产物（FDPs）也已显示出对细胞调节的作用。生长且与各种血管疾病有关。该协议旨在定量测量纤维蛋白分解的程度以及如何将其用作进一步研究纤维蛋白溶解或纤维蛋白降解产物的途径的工具。到目前为止，我们还没有发现了另一种分析纤维蛋白溶解的方法。

[背景 ] 在止血过程中，纤维蛋白既是纤维蛋白溶解的最终底物，又是凝血级联反应的主要产物（Cesarman-Maus 和Hajjar ，2005 ；Chapin和Hajjar，2015 ）。对植入的生物材料的急性炎性反应，已表明血纤蛋白在白细胞的迁移进而发炎中起着重要作用（Yakovlev and Medved ，2018）。血纤蛋白溶解是一个严格调节的过程，通过该过程，富含血纤蛋白的血栓形成和降解，防止血凝块的生长（Amengual 和Atsumi ，2016）并有助于组织修复（Houslay 等人，2019）。在纤维蛋白溶解过程中，纤维蛋白凝块（凝血产物）被分解，纤维蛋白降解产物（FDPs）被分解。作为促有丝分裂因子的纤维蛋白可以促进内皮细胞，平滑肌细胞和成纤维细胞的增殖以及胆固醇沉积（Dong ...

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