PE anti-mouse CD44 antibody

Company: BioLegend

Catalog#: 103023

Functional ex-vivo Imaging of Arterial Cellular Recruitment and Lipid Extravasation

Emiel P.C. van der Vorst, Sanne L. Maas, Almudena Ortega-Gomez, Jeroen M.M. Hameleers, Mariaelvy Bianchini, Yaw Asare, Oliver Soehnlein, Yvonne Döring, Christian Weber and Remco T.A. Megens

Published online: 2017-06-20

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Characterization of Immunological Niches within Peyer’s Patches by ex vivo Photoactivation and Flow Cytometry Analysis

Author:

Adi Biram and Ziv Shulman,

Date:

2020-03-20

[Abstract] T follicular helper (Tfh) cells regulate B cell selection for entry into the germinal center (GC) reaction or for differentiation into antibody forming cells. This process takes place at the border between the T and B zones in lymphoid organs and involves physical contacts between T and B cells. During these interactions, T cells endow the B cells with selection signals that promote GC seeding or plasmablast differentiation based on their B cell receptor affinity. In Peyer’s patches (PPs), T cells promote B cell colonization of the subepithelial dome (SED) without effective affinity-based clonal selection. To specifically characterize the T cell population that resides within the SED niche, we performed ex vivo photoactivation of the SED compartment followed by flow cytometry ... [摘要] [摘要] T卵泡辅助细胞（Tfh ）调节B细胞的选择，使其进入生发中心（GC）反应或分化为抗体形成细胞。此过程发生在淋巴器官的T和B区之间的边界，涉及T和B细胞之间的物理接触。在这些相互作用中，T细胞赋予B细胞选择信号，这些信号根据其B细胞受体亲和力促进GC接种或成浆细胞分化。在Peyer氏斑（PPs）中，T细胞在没有有效亲和力的情况下促进上皮下穹sub （SED）的B细胞定殖。 ed克隆选择。为了具体表征驻留在SED利基空间中的T细胞群体，我们按照本方案中所述对SED隔室进行了离体光激活，然后进行了标记细胞的流式细胞仪分析。该技术将空间和细胞信息整合到了免疫小生境研究中，可以适应各种实验系统。

【背景技术】亲和力成熟是血清抗体对特定抗原的亲和力随时间而增加的过程，这是通过在生发中心（GC）中选择带有高亲和力BCR的B细胞来实现的。增加抗体亲和力通过体细胞超突变和基于亲和力的选择，这由T滤泡辅助（编排的处理的反复循环介导的TFH ）细胞奥普雷亚;（开普勒和Perelson，1993 和Perelson，1997; Victora和Nussenzweig， 2012）。GC由两个微观解剖部位组成；暗区（B细胞增殖并获得体细胞超突变）和亮区（B细胞与相关抗原和T细胞相互作用）。尽管活体成像技术能够确定GC中的免疫细胞动力学（Allen 等，2007； ...

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PE anti-mouse CD44 antibody

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