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Centrifuge adapted for Flow cytometry tubes

Company: Eppendorf
Catalog#: Centrifuge 5810 R
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An ex vivo Approach to Assess Mitochondrial ROS by Flow Cytometry in AAV-tagged Astrocytes in Adult Mice
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2020-03-20
[Abstract]  Mitochondrial reactive oxygen species (mROS) are naturally produced signalling molecules extremely relevant for understanding both health- and disease-associated biological processes. The study of mROS in the brain is currently underway to decipher their physiopathological roles and contributions in neurological diseases. Recent advances in this field have highlighted the importance of studying mROS signalling and redox biology at the cellular level. Neurons are especially sensitive to the harmful effects of excess mROS while astrocytic mROS have been shown to play a relevant physiological role in cerebral homeostasis and behaviour. However, given the complexity of the brain, investigating mROS formation in a specific cell-type in adult animals is methodologically challenging. Here we ... [摘要]  [摘要] 线粒体活性氧(mROS )是天然产生的信号分子,与理解健康和疾病相关的生物过程极为相关。目前正在研究大脑中的mROS ,以了解其在神经疾病中的生理病理作用和贡献。该领域的最新进展凸显了在细胞水平研究mROS 信号传导和氧化还原生物学的重要性。神经元对过量的mROS 的有害作用特别敏感,而星形细胞的mROS对 已经显示出在脑稳态和行为中起相关的生理作用。但是,鉴于大脑的复杂性,研究成年动物中特定细胞类型中mROS的形成在方法上具有挑战性。在这里,我们提出了一种方法来具体评估星形胶质细胞中的mROS 丰度,该方法结合了i )一种靶向策略,该策略基于在星形胶质细胞(胶质纤维酸性蛋白或GFAP)下表达绿色荧光蛋白(GFP)的腺相关病毒(AAV)载体的使用)启动子,以及ii)使用商业探针通过流式细胞术测量mROS 的强大且广泛扩展的方案。这项工作的意义在于,它允许通过易于使用的技术来选择性研究星形细胞mROS的丰度。

[背景 ] 氧化损伤与许多疾病的病因有关,包括神经退行性疾病,因为大脑是氧化应激以及与年龄相关的变化异常脆弱的组织(Cobley 等,2018; Mattson和Arumugam ...

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