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TOPO® TA Cloning® Kit for Sequencing, with One Shot® TOP10 Chemically Competent E. coli

TOPO ® TA Cloning ® Kit for Sequencing,with One Shot ® TOP10化学感受态大肠杆菌

Company: Thermo Fisher Scientific
Catalog#: K457501
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Generation of Polyclonal Specific Antibodies
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2013-06-05
[Abstract]  Generation of antibodies specific for a protein of interest is a common method in many disciplines. This protocol details the steps in production of a polyclonal antibody in rabbits using a bacterially expressed fusion protein as an antigen. The protocol is generated based on data presented in Wirschell et al.(2013). [摘要]  对感兴趣的蛋白质特异性的抗体的产生是许多学科中的常见方法。 该方案详述了使用细菌表达的融合蛋白作为抗原在兔中产生多克隆抗体的步骤。 该协议基于Wirschell等人(2013)中提供的数据生成。

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