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SIGMAFAST BCIP®/NBT

SIGMAFASTTMBCIP® 20/ NBT

Company: Sigma-Aldrich
Catalog#: B5655
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Infection of Nicotiana benthamiana Plants with Potato Virus X (PVX)
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Date:
2016-12-20
[Abstract]  Potato Virus X (PVX) is the type member of Potexvirus genus, a group of plant viruses with a positive-strand RNA genome (~6.4 kb). PVX is able to establish compatible infections in Nicotiana benthamiana, a commonly used host in plant virology, leading to mild symptoms, such as chlorotic mosaic and mottling. PVX has been widely used as a viral vector for more than two decades (Chapman et al., 1992; Baulcombe et al., 1995; Aguilar et al., 2015). It provides a feasible means for the systemic expression in plants of heterologous proteins, such as avirulence factors, proteins with pharmacological properties, etc., (Hammond-Kosack et al., 1995; Gleba et al., 2014), and also as a tool to help decipher the function ... [摘要]  马铃薯病毒X(PVX)是Potexvirus属(一组具有正链RNA基因组(〜6.4kb)的植物病毒)的类型成员。 PVX能够在烟草烟草(Nicotiana benthamiana)中建立相容的感染,这是常用于植物病毒学的宿主,导致轻度症状,如褪色马赛克和斑驳。 PVX已经被广泛用作病毒载体二十多年(Chapman等人,1992; Baulcombe等人,1995; Aguilar等人。它为异源蛋白质植物中的全身表达提供了可行的手段,例如无毒性因子,具有药理学性质的蛋白质,等等(Hammond-Kosack等人, 1995; Gleba等人,2014),并且还作为通过病毒诱导的基因沉默(VACS)(Lacomme和Chapman,2008)帮助解释植物中基因功能的工具。摩擦(A)和农用过滤(B)的两种不同的方案,即允许PVX在N中的有效乘法和传播。本文详细描述本文。摩擦方法需要先前感染的液体,并且通过诱导对叶组织的机械损伤来实现感染,从而允许病毒颗粒穿透植物表面。农杆菌过滤需要先前修饰的农杆菌携带并将PVX序列递送到植物细胞中。将土壤杆菌生长直至饱和,并用注射器将其浸入植物组织中建立感染。这两种方法中的任何一种都可以成功应用,选择应主要基于材料和时间的可用性,但建议在使用嵌合病毒时使用农杆菌滤过。

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INF-gamma Release ELISpot Assay
Author:
Date:
2012-03-20
[Abstract]  IFN-γ (Interferon-gamma) is produced mainly by activated T cells and NK cells. Production of IFN-γ (Interferon-gamma) by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ (Interferon-gamma) is typically associated with effective host defense against intracellular pathogens. The Enzyme-Linked ImmunoSpot (ELISpot) assay is commonly used to assess the function of antigen specific T cells by detecting IFN-γ release. The ELISpot assay is a very sensitive immunoassay, allowing the detection of a secreted cytokine at the single cell level. With detection levels that can be as low as one cell in 100,000, the ELISpot is one of the most sensitive cellular assays available. Depending on the substance analyzed, the ELISpot assay ... [摘要]  119.txt

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