DMEM, powder, high glucose, pyruvate

DMEM，粉末，高葡萄糖，丙酮酸盐

Company: Thermo Fisher Scientific

Catalog#: 12800017

In vitro Glutamylation Inhibition of Ubiquitin Modification and Phosphoribosyl-Ubiquitin Ligation Mediated by Legionella pneumophila Effectors

Alan G. Sulpizio, Marena E. Minelli and Yuxin Mao

Published online: 2020-11-05

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Detection of HBV C Protein Phosphorylation in the Cell

Author:

Jaesung Jung and Kyongmin Kim,

Date:

2015-08-05

[Abstract] Among the seven serines and one threonine in the carboxyl-terminus of HBV C protein, all but one (serine 183) appear in the context of RxxS/T consensus phosphoacceptor motifs and also overlap with other consensus motifs, such as S/TP, RS, SPRRR, RRRS/T, or RRxS/T, suggesting that various cellular kinases phosphorylate these residues. To determine whether threonine and/or serine (serines 157, 164, 170, 172, 178, and 180, and threonine 162, adw subtype) of HBV C protein are indeed phosphoacceptor residues in cells, Huh7 were transfected with a series of C-protein-expressing mutants, labeled with ³²P-orthophosphate for 14 h, and then lysed. The ³²Pi-labeled lysates were immunoprecipitated with anti-HBc antibody, and the ³²Pi-labeled immunoprecipitated C ... [摘要] 在HBV C蛋白的羧基末端中的七个丝氨酸和一个苏氨酸中，除了一个（丝氨酸183）外，所有其它丝氨酸出现在RxxS/T共有磷酸受体基序的上下文中，并且还与其他共有基序重叠，例如S/TP，RS ，SPRRR，RRRS/T或RRxS/T，表明各种细胞激酶磷酸化这些残基。为了确定HBV C蛋白的苏氨酸和/或丝氨酸（丝氨酸157,164,170,172,178和180以及苏氨酸162，adw亚型）是否确实是细胞中的磷酸受体残基，Huh7用一系列C- 蛋白表达突变体，用正磷酸32P标记14小时，然后裂解。用抗HBc抗体免疫沉淀 32 P标记的裂解物，并通过放射自显影检测32 P标记的免疫沉淀的C蛋白。

Assay for Adherence of Vibrio cholerae to Eukaryotic Cell Lines

Author:

Amit K. Dey, Abha Bhagat and Rukhsana Chowdhury,

Date:

2014-04-20

[Abstract] Vibrio cholerae (V. cholerae) colonizes the intestinal epithelium and secretes cholera toxin (CT), a potent enterotoxin that causes severe fluid loss characteristic of the disease cholera. V. cholerae is a non-invasive Gram-negative bacterium that adheres to intestinal cells as well as a variety of different cell types. A protocol for adherence of V. cholerae to various cell lines is described. The adhered bacteria can be used to examine expression of genes that are differentially expressed between adhered and unadhered bacteria or other purposes (Dey et al., 2013).
[摘要] 霍乱弧菌（霍乱弧菌）殖民肠上皮并分泌霍乱毒素（CT），霍乱毒素（CT）是一种有效的肠毒素，其导致霍乱的特征性严重的流体损失。 V。霍乱菌是一种非侵入性革兰氏阴性细菌，其粘附于肠细胞以及各种不同的细胞类型。遵守 V的协议。霍乱等对各种细胞系的影响。粘附的细菌可用于检查在粘附和未粘附的细菌或其他目的之间差异表达的基因的表达（Dey等人，2013）。

Primary Culture of Cortical Neurons

Author:

Rieko Muramatsu and Toshihide Yamashita,

Date:

2013-04-20

[Abstract] Primary culture of neurons from cerebral cortex is a popular model to study neuronal function in vitro and to explore the molecular mechanism of neurite outgrowth in the developing and adult central nervous system. This protocol is for preparing a culture of cerebral cortical neurons from postnatal rodent brain (Muramatsu et al., 2012). One day after cell plating, we can observe neurite outgrowth by microscope. [摘要] 来自大脑皮质的神经元的原代培养是研究体外神经元功能的流行模型，并且研究发育和成人中枢神经系统中神经突生长的分子机制。该方案用于从产后啮齿动物脑制备大脑皮层神经元的培养物（Muramatsu等人，2012）。细胞铺板后一天，我们可以通过显微镜观察神经突生长。

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DMEM, powder, high glucose, pyruvate

DMEM，粉末，高葡萄糖，丙酮酸盐

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