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Nuclease S7 Micrococcal nuclease (MNase)

微球菌核酸酶

Company: Roche Diagnostics
Catalog#: 10107921001
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Micrococcal Nuclease (MNase) Assay of Arabidopsis thaliana Nuclei
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2013-04-05
[Abstract]  Micrococcal nuclease (MNase) is able to produce double-strand breaks within nucleosome linker regions. The efficiency of MNase digestion depends on the degree of chromatin compaction, being more easily digested the regions of less compacted chromatin. The MNase protocol described here can be used to asses changes in the chromatin structure of nuclei extracted from Arabidopsis seedlings. [摘要]  微球菌核酸酶(MNase)能够在核小体接头区域内产生双链断裂。 MNase消化的效率取决于染色质压实的程度,更容易消化较少压实的染色质的区域。 这里描述的MNase方案可以用于评估从拟南芥幼苗提取的核的染色质结构的变化。

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