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Anti-Histone H4 (acetyl K5) antibody

抗组蛋白H4(乙酰K5)抗体

Company: Abcam
Catalog#: ab51997
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Immunoblot Analysis of Histone H4 Acetylation and Histone H2A Phosphorylation in Candida albicans
Author:
Date:
2013-10-20
[Abstract]  Posttranslational modifications of histones are required for different processes including transcription, replication and DNA damage repair. This protocol describes the preparation of a whole-cell extracts for the fungal pathogen Candida albicans. Furthermore, the extract is used to detect lysine acetylation of histone H4 as well as serine 129 phosphorylation of histone H2A by immunoblot analysis.
[摘要]  包括转录,复制和DNA损伤修复的不同过程需要组蛋白的翻译后修饰。 该方案描述了真菌病原体白色念珠菌的全细胞提取物的制备。 此外,提取物用于通过免疫印迹分析检测组蛋白H4的赖氨酸乙酰化以及组蛋白H2A的丝氨酸129磷酸化。

Western Analysis of Histone Modifications (Aspergillus nidulans)
Author:
Date:
2013-04-05
[Abstract]  Western blotting allows for the specific detection of proteins and/or modifications of proteins by an antibody of interest. This protocol utilizes a crude nuclei extraction protocol for Aspergillus nidulans to enrich for histones and other nuclear proteins prior to gel electrophoresis. Post translational modifications of histones may then be easily detected. After electrophoresis, the selected antibodies are used to detect and quantify levels of the modifications of interest. [摘要]  蛋白质印迹允许由感兴趣的抗体特异性检测蛋白质和/或蛋白质的修饰。 该方案在凝胶电泳之前利用构巢曲霉的粗核提取方案来富集组蛋白和其它核蛋白。 然后可以容易地检测组蛋白的翻译后修饰。 电泳后,所选择的抗体用于检测和定量感兴趣的修饰的水平。

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