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Author:
Date:
2018-10-05
[Abstract] Planarians are freshwater flatworms, well known for their ability to regenerate a complete organism from any piece of their body. Furthermore, planarians are constantly growing and degrowing throughout their lives, maintaining a functional and proportioned body. These properties rely on the presence of a population of adult stem cells and on the tight control of their cell renewal, which is based on the balance between the proliferation of new cells and their differentiation, and the death of unnecessary cells. Due to the importance of these two processes in planarian biology, over the years, researchers have optimized molecular techniques to detect both cell proliferation and cell death in planarians. Here, we present the two main protocols currently used for cell death detection and ...
[摘要] 涡虫是淡水扁虫，因其能够从身体的任何部分再生完整的有机体而闻名。 此外，涡虫在其一生中不断生长和去除，保持功能和比例的身体。 这些特性依赖于成体干细胞群的存在以及对细胞更新的严格控制，其基于新细胞增殖与其分化之间的平衡以及不必要细胞的死亡。 由于这两个过程在涡虫生物学中的重要性，多年来，研究人员已经优化了分子技术，以检测涡虫中的细胞增殖和细胞死亡。 在这里，我们提出了目前用于细胞死亡检测和量化的两种主要方案：Caspase-3活性定量和TUNEL分析。
【背景】成体生物体中的细胞更新是基于三个过程的复杂机制：（a）通过细胞死亡消除选定的细胞; （b）通过细胞分裂取代已消除的细胞，通常涉及成体干细胞及其后代; （c）新生细胞的分化及其与先前存在的组织的整合（Pellettieri和Sanchez ...
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