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1.5ml Graduated Conical Tube, Natural Standard Screw Cap (Sterile)

Company: STARLAB
Catalog#: E1415-2231
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6-hydroxydopamine (6-OHDA) Oxidative Stress Assay for Observing Dopaminergic Neuron Loss in Caenorhabditis elegans
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2018-09-20
[Abstract]  The nematode Caenorhabditis elegans is a powerful genetic model that can be used to investigate neuronal death. Research using C. elegans has been crucial to characterize cell death programmes that are conserved in mammals. Many neuronal signaling components, such as those mediating dopaminergic neurotransmission, are preserved as well. Dopaminergic neurons are progressively lost in Parkinson’s disease and an important risk factor to develop this disease appears to be oxidative stress, the increased occurrence of highly reactive oxygen species. Oxidative stress-induced dopaminergic neurodegeneration is mimicked in animal models by treatment with 6-hydroxydopamine (6-OHDA), a dopamine analog, which is specifically taken up into dopaminergic neurons. After exposing C. ... [摘要]  线虫 Caenorhabditis elegans 是一种强大的遗传模型,可用于研究神经元死亡。使用 C进行研究。线虫对于表征哺乳动物中保守的细胞死亡程序至关重要。许多神经元信号传导组分,例如介导多巴胺能神经传递的组分,也被保留。多巴胺能神经元在帕金森病中逐渐丧失,并且发展该疾病的重要风险因素似乎是氧化应激,高活性氧物种的出现增加。通过用多巴胺类似物6-羟基多巴胺(6-OHDA)处理,在动物模型中模拟氧化应激诱导的多巴胺能神经变性,所述多巴胺类似物特异性地摄入多巴胺能神经元。暴露 C后。线虫到6-OHDA,可以很容易地监测荧光标记的多巴胺能神经元的丢失。生物体对氧化应激的敏感性被认为受基础内在氧化应激水平和抵抗氧化应激和氧化应激诱导的损伤的能力的影响。 C.线虫'6-OHDA模型'导致发现保护多巴胺能神经元所需的新基因,并且它有助于确定保守细胞死亡和细胞吞噬途径对多巴胺能神经变性的影响。在这里,我们描述了一个简单的协议,允许在 C中6-OHDA处理后容易检测多巴胺能神经元损失。线虫。

【背景】在暴露于氧化应激诱导药物6-羟基多巴胺(6-OHDA)后,可以在动物模型中概括多巴胺能神经元的逐渐丧失(综述Schober,2004)。与其他神经退行性药物如MPTP(1-甲基-4-苯基-1,2,3,6-四氢吡啶)相比,6-OHDA更安全,因为它不能通过血脑屏障。 ...

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