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Phenyl Sepharose High Performance

Phenyl Sepharose高性能

Company: Cytiva
Catalog#: 17-1082-01
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Purification of Antigen 85 Complex of Mycobacterium tuberculosis
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2013-02-05
[Abstract]  Serodiagnosis of tuberculosis using purified native antigens is one of the approaches for the early detection of TB. The Antigen 85 complex consisting of Ag 85A, 85B and 85C are the most abundant antigens in the culture supernatant of mycobacteria. It has also been shown to be the immunodominant (the property of an antigenic determinant that causes it to be responsible for the major immune response in a host) antigens of CFA (culture filtrate antigens – proteins secreted by mycobacteria into the culture medium). This protocol gives the details for purification of the Ag85 complex from CFA and also the method of isolation of the individual components of the complex by column chromatography. [摘要]  使用纯化的天然抗原的结核病的血清诊断是早期检测TB的方法之一。 由Ag 85A,85B和85C组成的抗原85复合物是分枝杆菌培养物上清液中最丰富的抗原。 它也被证明是CFA(培养物滤液抗原 - 分枝杆菌分泌到培养基中的蛋白质)抗原的免疫显性(导致其负责宿主主要免疫应答的抗原决定簇的性质)。 该方案给出了从CFA纯化Ag85复合物的细节以及通过柱色谱法分离复合物的各个组分的方法。

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