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Author:
Date:
2018-09-05
[Abstract] The striatum is located in the subcortical region of the forebrain, it contains medium spiny neurons, cholinergic interneurons and GABAergic interneurons, and receives dopaminergic projection in the nigrostriatal pathway. This protocol provides a method to collect synaptosomes from mouse brain dorsal stratum. The synaptosomes can be used to study dopamine uptake activity, dopaminergic terminal endocytosis/trafficking using biochemical and microscopy methods, and protein analysis (Sorkina et al., 2018).
[摘要] 纹状体位于前脑的皮质下区域，它包含中等刺状神经元，胆碱能中间神经元和GABA能中间神经元，并在黑质纹状体通路中接受多巴胺能投射。 该方案提供了从小鼠脑背侧层收集突触体的方法。 突触体可用于研究多巴胺摄取活性，使用生物化学和显微镜方法的多巴胺能末端内吞作用/运输，以及蛋白质分析（Sorkina 等，，2018）。
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