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Author:
Date:
2018-09-05
[Abstract] Glioma stem cells (GSC) grown as neurospheres exhibit similar characteristics to neural stem cells (NSC) grown as neurospheres, including the ability to self-renew and differentiate. GSCs are thought to play a role in cancer initiation and progression. Self-renewal potential of GSCs is thought to reflect many characteristics associated with malignancy, including tumor recurrence following cytotoxic therapy due to their proliferative dormancy and capacity to allow for the development of resistant tumor cell sub-clones due to mutations acquired during their differentiation. Here, we demonstrate that using extreme limiting dilution analysis (ELDA), subtle differences in the frequency of sphere-forming potential between PI3K-mutant oncogenic NSCs and non-oncogenic NSCs can be measured, in ...
[摘要] 作为神经球生长的神经胶质干细胞（GSC）表现出与作为神经球生长的神经干细胞（NSC）相似的特征，包括自我更新和分化的能力。 GSC被认为在癌症的发生和发展中起作用。 GSC的自我更新潜力被认为反映了与恶性肿瘤相关的许多特征，包括细胞毒性治疗后的肿瘤复发，这是由于它们的增殖性休眠和由于在其分化期间获得的突变而允许产生抗性肿瘤细胞亚克隆的能力。在这里，我们证明使用极限稀释分析（ELDA），可以测量PI3K-突变致癌NSCs和非致癌NSCs之间的球形成潜力频率的细微差异体外。我们进一步展示了ELDA如何在强制分化之前和之后用于细胞，以放大突变体和对照NSCs之间的球形成潜力的固有差异。最终，ELDA利用单个或少数种子干细胞自我更新，分裂和形成神经球的能力差异。重要的是，该测定还允许在不同条件下在遗传上不同的细胞之间或相同细胞之间进行比较，其中可以测试靶特异性药物或其他新型癌症干细胞疗法的影响。
【背景】胶质母细胞瘤（GBM）是最常见的脑癌之一，预后极差（Kaye和Morokoff，2014）。 ...
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