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Company: OHAUS
Catalog#: AR1140
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Generation of Fusarium graminearum Knockout Mutants by the Split-marker Recombination Approach
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2018-08-20
[Abstract]  Fusarium graminearum is a destructive phytopathogen and shows an impressive metabolic diversity. Gene deletion is an important and useful approach for gene function study. Here we present a protocol for generating gene deletion mutant by applying “split-marker” deletion strategy (Catlett et al., 2003) with PEG-mediated protoplast transformation (Yuan et al., 2008; Martín, 2015). [摘要]  禾谷镰刀菌是一种破坏性的植物病原体,具有令人印象深刻的代谢多样性。 基因缺失是基因功能研究的重要且有用的方法。 在这里,我们提出了一个协议,通过应用“分裂标记”删除策略(Catlett et al。,2003)与PEG介导的原生质体转化(Yuan 等。,2008;Martín,2015)。

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