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Streptavidin−Agarose from Streptomyces avidinii

来自Streptomyces avidinii的链霉亲和素 - 琼脂糖

Company: Sigma-Aldrich
Catalog#: S1638
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Co-Streptavidin Precipitation
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2013-02-05
[Abstract]  Co-Streptavidin Precipitation (Co-SP) is a method to pull down protein partners of a protein of interest tagged with the streptavidin binding protein domain, and using streptavidin columns that specifically bind to Streptavidin Binding Protein (SBP) in order to test the protein-protein interactions. Proteins of interest to be tested for their interaction are artificially co-expressed in “easy to transfect” cells. Pull down proteins can be analyzed by western blot for suspected protein partner. [摘要]  共链霉亲和素沉淀(Co-SP)是一种拉下用链霉亲和素结合蛋白结构域标记的目的蛋白质的蛋白质配偶体,并使用特异性结合链霉亲和素结合蛋白(SBP)的链霉亲和素柱以测试蛋白质的方法 蛋白相互作用。 要测试其相互作用的感兴趣的蛋白质在"易于转染"细胞中人工共表达。 下拉蛋白可以通过蛋白质印迹分析用于可疑蛋白质伴侣。

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