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Company: Wako Pure Chemical Industries
Catalog#: 345-02225
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Microscopic Observation of Subcellular GFP-tagged Protein Localization in Rice Anthers
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2018-08-05
[Abstract]  This protocol demonstrates a simple method to determine the subcellular localization of fluorescence-tagged proteins on the vibratome sections of rice developing anthers. If a cell type-specific promoter is used to drive the tagged protein-encoding gene, the method enables to clearly distinguish the cells retaining fluorescent signals from other anther cells. It is applicable to both live and fixed samples, and presumably to other plant tissues. [摘要]  该方案演示了一种确定荧光标记蛋白在水稻发育花药的振动切片上的亚细胞定位的简单方法。 如果使用细胞类型特异性启动子来驱动标记的蛋白质编码基因,该方法能够清楚地区分保留荧光信号的细胞与其他花药细胞。 它适用于活的和固定的样品,并且可能适用于其他植物组织。

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