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Sonicator Bath
Company: SND
Catalog#: US-101
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Measuring Secretion of Capsidiol in Leaf Tissues of Nicotiana benthamiana
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2018-08-05
[Abstract]  Plant species produce a wide variety of antimicrobial metabolites to protect themselves against potential pathogens in natural environments. Phytoalexins are low molecular weight compounds produced by plants in response to attempted attacks of pathogens. Accumulation of phytoalexins in attacked plant tissues can inhibit the growth of penetrating pathogens. Thus phytoalexins play a major role in post-invasion defense against pathogens. Major phytoalexins produced by Solanaceous plants are sesquiterpenoids such as capsidiol produced by Nicotiana and Capsicum species, and rishitin produced by Solanum species, which are synthesized in the cytosol and secreted into the intercellular space of plant tissues. We previously reported that deficiency in capsidiol secretion ... [摘要]  植物物种产生多种抗微生物代谢物，以保护自身免受自然环境中潜在的病原体的侵害。植物毒素是由植物响应于病原体的企图攻击而产生的低分子量化合物。植物抗毒素在受攻击的植物组织中的积累可以抑制穿透性病原体的生长。因此，植物抗毒素在入侵后对抗病原体的防御中起主要作用。茄科植物产生的主要植物抗毒素是倍半萜类化合物，如由 Nicotiana 和 Capsicum 物种产生的衣壳菌素，以及由 Solanum 物种产生的rishitin，其在胞质溶胶分泌到植物组织的细胞间隙中。我们以前曾报道，衣壳菌素分泌不足会导致本塞姆氏烟草对马铃薯晚疫病菌致病疫霉的敏感性增加。在这里，我们描述了一种测量 N中分泌的衣壳二醇的实用方案。本塞姆氏

【背景】该方案提供了一种快速简便的方法来量化衣壳菌醇的分泌，如Shibata 等（2016）所述。 因为环己烷通常用作洗去花粉层的溶剂，但能保持花粉活力（Doughty et al。，1993），我们开发了这种方法从叶子表面洗去分泌的代谢物来量化细胞外capsidiol。 可以使用该方法分析质膜局部转运蛋白的功能，同时还允许其与其他方法结合使用，例如使用质膜囊泡的生化转运分析（例如，Sugiyama 等人，，2007），以确定所检查的转运蛋白的底物。

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