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Author:
Date:
2018-07-20
[Abstract] Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones that were isolated in the screen.
This is a simple protocol to extract plasmid DNA from budding yeast cultures (Robzyk and Kassir, 1992). The amount produced is small, but it is sufficient for PCR or for transformation into bacteria, where the plasmid can be amplified to provide sufficient amounts for downstream uses (e.g., restriction enzyme analysis, sequencing).
[摘要] 质粒是酵母研究中广泛使用的工具。 在许多情况下，质粒文库用于遗传筛选或酵母双杂交筛选。 在这种情况下，有必要从筛选中分离的阳性克隆中提取携带未知遗传元件的质粒。
这是从芽殖酵母培养物中提取质粒DNA的简单方案（Robzyk和Kassir，1992）。 产生的量很小，但它足以用于PCR或转化到细菌中，其中质粒可以被扩增以提供足够的量用于下游用途（例如，，限制酶分析，测序）。
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