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Cobalt(II) chloride hexahydrate

Company: Sigma-Aldrich
Catalog#: C2911
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Micropropagation of Prickly Pear by Axillary Shoot Proliferation
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2018-07-05
[Abstract]  A protocol for the axillary bud proliferation of prickly pear (Opuntia; Cactaceae) is presented. This genus is widely used as a crop in the arid and semi-arid areas of the globe worldwide, providing numerous benefits for human and animal consumption. In vitro culture for axillary bud proliferation is of great use to obtain a large quantity of plants in a short period of time, with potential uses in production and for the preservation of endangered species of the Opuntia genus.

The optimal medium for Opuntia in vitro culture consists of Murashige and Skoog medium (MS) and L2 vitamins. To increase the yield of the axillary bud proliferation, we recommend the addition of plant growth regulators (PGRs). This work suggests a 15 d incubation in the ...
[摘要]  提出了刺梨( Opuntia; Cactaceae )腋芽增殖的方案。 该属广泛用作全球干旱和半干旱地区的作物,为人类和动物消费提供了许多益处。 腋芽增殖的体外培养对于在短时间内获得大量植物具有重要意义,可用于生产和保存罂粟的濒危物种 属。

仙人掌体外培养的最佳培养基由Murashige和Skoog培养基(MS)和L2维生素组成。 为了增加腋芽增殖的产量,我们建议添加植物生长调节剂(PGRs)。 这项工作表明在含有2.2mg / L苄基氨基嘌呤(BA)的培养基中培养15天,然后将外植体转移到不含PGR的培养基中。 我们还解释了如何使植物适应体外条件。

【背景】仙人掌(刺梨)属是仙人掌科家族的成员之一(Bravo-Hollis,1978)。虽然它原产于美洲,但目前生长在欧洲南部,非洲北部,澳大利亚,中东,西亚和世界其他地区的野生和商业种植园中(Ochoa和Barbera,1995; Kiesling和Metzing) ,2017)。仙人掌对干旱和半干旱环境以及生活在这些地区的人类群落产生了深远的影响,因为尽管在干旱地区生长,其生物量仍然很高(Acevedo et al。,1983)。在许多这样的领域, Opuntia 属被用于许多方面。 仙人掌的幼枝可以作为蔬菜食用;水果直接食用或加工成果冻,果汁或糖果(Barba et ...

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