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Water-saturated Phenol

Company: AMRESCO
Catalog#: 0981
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Phenol-based Extraction of RNA from Chlamydomonas reinhardtii
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2018-07-05
[Abstract]  RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in Chlamydomonas. However, in some cases RNA quality and quantity may be dramatically reduced depending on the strains and/or the conditions where cells were grown or treated. Phenol-based protocols are the most robust methods to obtain both high quantity and quality RNA from any strain of this alga grown in any condition. Here, we describe an easy and cheap protocol using phenol but avoiding the acute toxicity of guanidine isothiocyanate present in the commercial phenol-based mixtures. [摘要]  RNA提取是分子生物学中的基本程序,并且可获得各种商业试剂盒。 其中一些试剂盒已成功用于 Chlamydomonas 。 然而,在一些情况下,取决于菌株和/或细胞生长或处理的条件,RNA质量和数量可以显着降低。 基于苯酚的方案是从任何条件下生长的这种藻类的任何菌株获得高数量和高质量RNA的最稳健的方法。 在这里,我们描述了使用苯酚的简单且廉价的方案,但避免了商业苯酚基混合物中存在的异硫氰酸胍的急性毒性。

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