Sucrose, Ultra Pure Grade,1kg

Company: First BASE Laboratories

Catalog#: BIO-1090-1kg

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Dissecting the Mechanical Control of Mitotic Entry Using a Cell Confinement Setup

Margarida Dantas, Débora Vareiro and Jorge G. Ferreira

Published online: 2024-03-20

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A Method for Extracting the Nuclear Scaffold from the Chromatin Network

Author:

Junjie Chen, Boon Heng Dennis Teo and Jinhua Lu,

Date:

2018-04-20

[Abstract] Each cell contains many large DNA polymers packed in a nucleus of approx. 10 μm in diameter. With histones, these DNA polymers are known to form chromatins. How chromatins further compact in the nucleus is unclear but it inevitably depends on an extensive non-chromatin nuclear scaffold. Imaging of endogenous chromatin network and the complementary scaffold that support this network has not been achieved but biochemical and proteomic investigations of the scaffold can still provide important insights into this chromatin-organizing network. However, this demands highly inclusive and reproducible extraction of the nuclear scaffold. We have recently developed a simple protocol for releasing the scaffold components from chromatins. The inclusiveness of the extract was testified by the ... [摘要] 每个细胞都含有许多大型DNA聚合物，其中包含大约一个核。直径10微米。用组蛋白，已知这些DNA聚合物形成染色质。染色质在核中如何进一步致密还不清楚，但它不可避免地依赖于广泛的非染色质核支架。内源性染色质网络的成像和支持该网络的互补支架尚未实现，但支架的生化和蛋白质组学研究仍然可以提供关于该染色质组织网络的重要见解。但是，这需要高度包容和可重复的提取核支架。我们最近开发了一个简单的协议，用于从染色质中释放脚手架组件。提取物的包容性由以下观察结果证实：当从核中提取时，剩余的核染色质被释放为延伸且通常平行的染色质纤维。基本上，该方案包括纯核的产生，用Triton X-100处理细胞核以产生包膜消耗的细胞核（TxN），并在含蔗糖的缓冲液中在500mM NaCl中提取细胞核。 TxN的这个组合提取被称为TxNE。

【背景】通过蛋白质和核糖核蛋白的复杂支架，染色质在细胞核中密集并动态地压缩。与细胞骨架网络不同（Fischer和Fowler，2015），对这种核支架的显微观察在技术上是具有挑战性的。这可能反映了每个细胞核内染色质的主导地位，支架与细胞核交织在一起。核的球形排列也对成像这种支架结构造成挑战。核支架的主要元素是核层（NL）（Gruenbaum和Foisner，2015）。 ...

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Sucrose, Ultra Pure Grade,1kg

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