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Microwave

Company: Moulinex
Catalog#: Micro-chef FM2515Q
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Quantification of Thrips Damage Using Ilastik and ImageJ Fiji
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2018-04-20
[Abstract]  Quantification of insect damage is an essential measurement for identifying resistance in plants. In screening for host plant resistance against thrips, the total damaged leaf area is used as a criterion to determine resistance levels. Here we present an objective novel method for analyzing thrips damage on leaf disc using the freely available software programs Ilastik and ImageJ. The protocol was developed in order to screen over 40 Capsicum lines for resistance against Frankliniella occidentalis (Western Flower Thrips) and Thrips tabaci (Onion thrips). [摘要]  昆虫损伤的定量是鉴定植物抗性的重要指标。 在筛选宿主植物对蓟马的抗性时,将总的受损叶面积用作确定抗性水平的标准。 在这里我们提出了一种客观的新方法,用免费提供的软件程序Ilastik和ImageJ来分析叶盘上的蓟马损伤。 该协议的开发是为了筛选超过40个辣椒品系对西方花蓟马和西蓟马蓟马(洋葱蓟马)的抗性。

【背景】昆虫损伤的定量是鉴定植物抗性的重要指标。在筛选宿主植物对蓟马抗性的程序中,总损伤叶面积被用作确定抗性水平的标准。蓟马损害的特点是银色斑点与完整叶片区域显示出高对比度,但喂食点还包括从深绿色到棕色的深色区域。这些叶片的逐渐变色太细微,无法用诸如Winfolia等程序精确量化( http:// www .regentinstruments.com / assets / winfolia_software.html )或ImageJ(Rasband,2011)。结果,蓟马损害通常由评估样品的个体评分。将样本分为表示损害数量的类别(Mirnezhad等人,2010; Maharijaya等人,2011和2012),或损害估计为最接近的1 (Leiss等人,2009; ...

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