Fisherbrand^TM SureOne^TM Filter Tip Reload, Extended Length Pipette Tips

Company: Fisher Scientific

Catalog#: 02-707-474

Purification of Native Dentilisin Complex from Treponema denticola by Preparative Continuous Polyacrylamide Gel Electrophoresis and Functional Analysis by Gelatin Zymography

Pachiyappan Kamarajan, John C. Timm, M. Paula Goetting-Minesky, Erin T. Malone, Sean Ganther, Allan Radaic, Christian Tafolla, J. Christopher Fenno and Yvonne L. Kapila

Published online: 2024-04-05

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Isolation of Commensal Escherichia coli Strains from Feces of Healthy Laboratory Mice or Rats

Author:

Tingting Ju and Benjamin P. Willing,

Date:

2018-03-20

[Abstract] The colonization abundance of commensal E. coli in the gastrointestinal tract of healthy laboratory mice and rats ranges from 10⁴ to 10⁶ CFU/g feces. Although very well characterized, the family that E. coli belongs to has a very homogeneous 16S rRNA gene sequence, making the identification from 16S rRNA sequencing difficult. This protocol provides a procedure of isolating and identifying commensal E. coli strains from a healthy laboratory mouse or rat feces. The method can be applied to isolate commensal E. coli from other laboratory rodent strains. [摘要] 共生E的殖民丰度。大肠杆菌在健康实验小鼠和大鼠的胃肠道中的范围为10 4至10 6 CFU / g粪便。虽然描述得非常好，但那个家族就是这样的。大肠杆菌属于具有非常均一的16S rRNA基因序列，使得从16S rRNA测序鉴定困难。该协议提供了分离和识别共生E的程序。来自健康实验室小鼠或大鼠粪便的大肠杆菌菌株。该方法可以应用于隔离共生电子。来自其他实验室啮齿类动物的大肠杆菌。

【背景】大肠杆菌是革兰氏阴性兼性厌氧菌，其仅构成脊椎动物肠道微生物群的一小部分，但在微生物相互作用，免疫调节和代谢功能中起关键作用（Tenaillon等人。，2010）。作为最好的模式微生物之一，共生E。已经越来越多地研究大肠杆菌菌株以揭示肠道共生微生物适应独特生态位并影响宿主生理机制。然而，不同菌株之间的高度同源性在共生E的鉴定和表征上提出了困难。基于16S rRNA测序方法的大肠杆菌。由于新一代测序技术的发展和全基因组的大规模分析，我们能够识别共生E。根据基因组中毒力基因的存在，分离自不同宿主的胃肠道的大肠杆菌菌株。在这个协议中，我们展示了一种分离和识别共生E的方法。使用选择性培养基和全基因组测序从实验室小鼠或大鼠获得大肠杆菌菌株。但是，应该指出的是，共生E的存在。大肠杆菌在实验室动物中取决于设施的供应商和环境条件。

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