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Company: C&A Scientific
Catalog#: 97-5313
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Large Scale Field Inoculation and Scoring of Maize Southern Leaf Blight and Other Maize Foliar Fungal Diseases
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2018-03-05
[Abstract]  Field-grown maize is inoculated with Cochliobolus heterostrophus, causal agent of southern leaf blight disease, by dropping sorghum grains infested with the fungus into the whorl of each maize plant at an early stage of growth. The initial lesions produce secondary inoculum that is dispersed by wind and rain, causing multiple cycles of infection that assures a high uniform disease pressure over the entire field by the time of disease scoring, which occurs after anthesis. This method, with slight modifications, can also be used to study the maize fungal diseases northern leaf blight (caused by Exserohilum turcicum) and gray leaf spot (Cercospora zeae-maydis). [摘要]  田间种植的玉米接种南方叶枯病的致病剂异叶蜗杆菌,通过在生长的早期阶段,将侵染了真菌的高粱谷物滴入每个玉米植株的螺纹中。 最初的病变产生由风和雨分散的二次接种物,引起多次感染循环,从而在开花后发生疾病评分时在整个区域确保高度均匀的疾病压力。 这种方法只需稍作修改,也可用于研究北方叶枯病(由Exserohilum turcicum引起)和灰叶斑点(Emesospora zeae-maydis)引起的玉米真菌病害。


【背景】南方叶枯病(SLB),由蜗杆异轴旋回(Drechs。)Drechs引起。 [anamorph = Bipolaris maydis (Nisikado)Shoemaker]是一种普遍的玉米病害,在热带,潮湿的热带和亚热带地区,如美国东南部,印度部分地区,非洲,拉丁美洲和南欧。在1970 - 71年,由C引起的SLB流行。异质性种群T感染了携带德克萨斯雄性不育细胞质(cms-T)的杂交种,导致美国总玉米产量估计损失15%(Ullstrup,1972)。在1970年流行后,cms-T玉米被抗T种质的正常细胞质玉米所取代。

目前,O型是美国和全球SLB的主要原因(Wang等人,2017)。 SLB抵抗 C。 heterostrophus race O是主要由加性或部分显性基因作用定量遗传的(Holley and ...

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