Author:
Date:
2018-01-20
[Abstract] There are some bacteria which can grow and multiply at the cost of living fungal biomass. They can potentially utilize fungi as a source of nutrients to forage over them. Such phenomenon is known as bacterial mycophagy, however, its mechanistic insights need to be explored to identify the molecules involved in mycophagy for potential utilization in controlling various fungal diseases. Recently we have demonstrated that a rice-associated bacteria Burkholderia gladioli strain NGJ1 exhibits mycophagous ability on several fungi, including Rhizoctonia solani, the necrotrophic fungal pathogen causing sheath blight disease in rice. We hereby describe our validated and efficient methods used to study B. gladioli strain NGJ1-R. solani interactions. These ...
[摘要] 有一些细菌可以生长和繁殖以牺牲真菌生物量为代价。 他们可能潜在地利用真菌作为营养素的来源来搜寻它们。 这种现象被称为细菌真菌病,然而,其机理性的见解需要被探索,以确定参与mycophagy分子潜在的利用控制各种真菌病害。 最近我们已经证明,稻米相关的细菌伯克霍尔德氏唐菖蒲菌株NGJ1在几种真菌上展现出真菌的能力,包括水稻纹枯病真菌病原体,即立枯丝核菌(Rhizoctonia solani)。 我们在此描述我们用于研究B的已验证的和有效的方法。 剑兰菌株NGJ1- R。 solani 互动。 这些方法学可用于设计分析来研究细菌和真菌之间的对抗,从而能够从这些细菌中发现新的抗真菌分子。
【背景】立枯丝核菌(Rhizoctonia solani)是具有多种宿主的重要的植物病原真菌。导致稻纹枯病;在爆发性疾病之后,稻米的第二大破坏性真菌病(Fisher等人,2012; Ghosh等人,2014; Ghosh等人, >,2017)。在发展水稻纹枯病防治方法之后,我们从水稻幼苗中分离出一株具有广谱抗真菌活性的细菌。基于rDNA和基因组测序,该细菌已经被鉴定为唐菖蒲伯克霍尔德氏菌菌株NGJ1(Jha等人,2015)。除了抗真菌特性外,我们观察到NGJ1具有对真菌进行觅食的能力,并且在禾本科植物上表现出食菌能力。 solani 以及其他各种真菌。在与 R交互之后。 ...
|
Author:
Date:
2018-01-05
[Abstract] Lipopeptides is an important class of biosurfactants having antimicrobial and anti-adhesive activity against pathogenic bacteria. These include surfactin, fengycin, iturin, bacillomycin, mycosubtilin, lichenysin, and pumilacidin (Arima et al., 1968; Naruse et al., 1990; Yakimov et al., 1995; Steller and Vater, 2000; Roongsawang et al., 2002; Vater et al., 2002). To date, none of these lipopeptides have been reported to possess any anti-motility activity. We isolated, purified and characterized two novel cyclic lipopeptides (CLPs) from Bacillus sp. 176 using high performance liquid chromatography, mass spectrometry and nuclear magnetic resonance spectroscopy. CLPs dramatically suppress the motility of pathogenic bacterium Vibrio ...
[摘要] 脂肽是一类重要的生物表面活性剂,对致病菌具有抗菌和抗粘连活性。这些包括表面活性肽,fengycin,伊库菌素,杆菌霉素,mycosubtilin,地衣素和pumilacidin(Arima等人,1968; Naruse等人,1990; Yakimov等人1995; Steller和Vater,2000; Roongsawang等人,2002; Vater等人,2002)。迄今为止,这些脂肽都没有被报道具有任何抗运动活性。我们从芽孢杆菌分离,纯化和鉴定了两种新的环状脂肽(CLPs)。 176使用高效液相色谱,质谱和核磁共振光谱。 CLPs极大地抑制致病性溶藻弧菌的运动,并促进细胞聚集而不诱导细胞死亡。根据Dalili所述用于抗生物膜测定的方法进行细胞聚集测定(Dalili等人,2015)。将来,该测定法可以适用于测试来自细菌的脂肽样活性物质的细胞聚集和抗生物膜活性。
【背景】过度使用广谱抗生素和随之而来的抗药性细菌的增殖刺激了开发环境友好型生物防治措施的努力,以减少健康危害和环境污染(Nam等人,2016; Sajitha >等。,2016)。近年来,生物表面活性剂的抗微生物特性已被越来越多地用于抗菌,抗真菌和抗病毒的应用(Cameotra和Makkar,2004; Singh和Cameotra,2004; Rodrigues等人 ...
|