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Gilson pipettes

Company: Scientific Laboratory Supplies
Catalog#: F123600
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Surgical Induction of Endometriosis in Female Mice
Author:
Date:
2020-09-20
[Abstract]  Endometriosis is a common gynecological disease characterized by the presence of endometrial tissue outside the uterine cavity. It is frequently associated with pain, infertility and a reduced quality of life, and it lacks adequate treatment. Several rodent models of endometriosis have been developed through heterologous and homologous transplantation of endometrial tissue into the abdominal compartment. Here we describe a surgical procedure to generate a syngeneic model of endometriosis in immunocompetent mice with intact uterine and ovarian tissues. In this model, four uterine fragments from a donor mouse at diestrus are sutured to the abdominal wall of a recipient mouse. One month after surgeries, endometrial implants develop into cysts with glandular epithelium and stroma, mimicking ... [摘要]  [摘要 ] 子宫内膜异位症是一种常见的妇科疾病,其特征是子宫腔外存在子宫内膜组织。它经常与疼痛,不育和生活质量下降有关,并且缺乏适当的治疗。子宫内膜异位症的几种啮齿动物模型是通过将子宫内膜组织异源和同源移植到腹腔中而开发的。在这里,我们描述了在具有完整子宫和卵巢组织的免疫功能小鼠中产生子宫内膜异位症的同基因模型的手术程序。在该模型中,将来自供体小鼠在二头肌的四个子宫碎片缝合到受体小鼠的腹壁上。手术一个月后,子宫内膜植入物发展成具有腺上皮和间质的囊肿,模仿了子宫内膜异位症女性中观察到的子宫内膜异位病变。因此,该小鼠模型为研究子宫内膜异位症的病理生理学和潜在治疗方法的有效性提供了有价值的工具。

[背景 ] 子宫内膜异位是由子宫外子宫内膜组织的生长所定义的一种慢性妇科疾病,主要在骨盆和腹部表面生长(Zondervan et al。,2018)。它影响了育龄妇女的10%,并且与疼痛,不孕症和生活质量下降有关(Fourquet 等人,2011;Márki 等人,2017; Zondervan 等人,2018)。子宫内膜异位症的可用药物和手术疗法具有不良作用,并且无法长期缓解症状(Falcone和Flyckt,2018年)。因此,需要新的治疗策略,其发展依赖于动物模型的建立,该模型概括了临床子宫内膜异位症的病理生理和行为特征。

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Microtubule Seeded-assembly in the Presence of Poorly Nucleating Nucleotide Analogues
Author:
Date:
2020-08-20
[Abstract]  Microtubule dynamic instability is driven by the hydrolysis of the GTP bound to the β-subunit of the α-β tubulin heterodimer. Nucleotide analogues are commonly used to mimic the different steps of the tubulin GTPase cycle, but most of them are poor microtubule nucleators. Usually, microtubule assembly is seeded by guanylyl-(α, β)-methylene-diphosphonate (GMPCPP) or glycerol that can be limiting factors in monitoring the effect of other nucleotide analogs on their polymerization. Here, we describe a protocol that allows the assembly of microtubules in the presence of nucleotide analogues without the need of heterogeneous seeds and at a low final glycerol concentration. Microtubules are first assembled in the presence of the analogue of interest and glycerol to promote assembly. These ... [摘要]  [摘要 ] 微管动态不稳定性是由与α - β 微管蛋白异二聚体的β- 亚基结合的GTP水解驱动的。核苷酸类似物通常用于模拟微管蛋白GTPase循环的不同步骤,但其中大多数是不良的微管成核剂。通常,微管组装是通过胍基-(α ,β )-亚甲基-二膦酸酯(GMPCPP)或甘油来接种的,它们可能是限制其他核苷酸类似物对其聚合作用的监测因素。 在这里,我们描述了一种协议,该协议允许在核苷酸类似物存在的情况下组装微管,而无需异质种子,并且最终甘油浓度低。首先在感兴趣的类似物和甘油的存在下组装微管以促进组装。然后对这些微管进行超声处理,以产生种子,这些种子将在不存在甘油的情况下用于组装微管。这种策略产生了均质的核苷酸结合的微管,可以通过生化或结构方法(如冷冻电子显微镜)进一步分析。

[背景 ] 核苷酸类似物通常用于研究的构象变化,该α - β 微管组装和水解绑定到其可交换的(E)的网站上的GTP的期间微管蛋白异源二聚体经历。但是,除GMPCPP以外,大多数类似物都是微管成核剂。为克服这一困难,GMPCPP稳定化的种子通常用于存在其他类似物的情况下延长微管(Maurer 等人,2011和2014; Zhang ...

Stopped-flow Light Scattering Analysis of Red Blood Cell Glycerol Permeability
Author:
Date:
2020-08-20
[Abstract]  Stopped-Flow Light Scattering (SFLS) is a method devised to analyze the kinetics of fast chemical reactions that result in a significant change of the average molecular weight and/or in the shape of the reaction substrates. Several modifications of the original stopped-flow system have been made leading to a significant extension of its technical applications. One of these modifications allows the biophysical characterization of the water and solute permeability of biological and artificial membranes.

Here, we describe a protocol of SFLS to measure the glycerol permeability of isolated human red blood cells (RBCs) and evaluate the pharmacokinetics properties (selectivity and potency) of isoform-specific inhibitors of AQP3, AQP7 and AQP9, three mammalian aquaglyceroporins ...
[摘要]  [摘要] 停流光散射(SFLS)是一种用于分析快速化学反应动力学的方法,该化学反应导致平均分子量和/或反应底物形状发生显着变化。已对原始停流系统进行了几处修改,从而大大扩展了其技术应用范围。这些修饰之一允许水的生物物理表征以及生物膜和人造膜的溶质渗透性。

在这里,我们描述了一种SFLS协议,用于测量分离的人红细胞(RBC)的甘油渗透性,并评估AQP3,AQP7和AQP9的同种型特异性抑制剂的药代动力学特性(选择性和效价),这三种哺乳动物的水甘油糖蛋白允许转运甘油跨膜。在20°C的SFLS设备中,将RBC的悬浮液(1%的血细胞比容)暴露于100 mM甘油的向内定向,并在530 nm的单色波长下记录120 s的散射光强度变化。该设备的死区时间为1.6毫秒,混合效率在不到1毫秒的时间内达到99%。数据拟合到单指数函数和有关的时间constan 吨( ,对应于伴随甘油的进入红细胞的水渗透运动的光散射的细胞膨胀相的秒)进行测定。RBC 的甘油渗透系数(P gly ,cm / s)通过以下公式计算:



P gly = 1 / [[((S / V) ]



其中 (s)是拟合的指数时间常数,S / V是分析的RBC 样本的表面积与体积之比(cm -1 ...

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