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Author:
Date:
2017-11-20
[Abstract] Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael et al., 1987). In this study, the protocol is being used to determine the cell viability of plant roots, treated with different stress inducing agents. The basis of the assay is that the dye enters the living cell’s mitochondrion where it is reduced to insoluble formazan, which is solubilised by directly treating the cells with organic solvent (DMSO). Intensity of colour is directly proportional to the amount of formazan produced.
In the present study, plants were treated for 16 h, with several phytotoxic agents, then the roots were incubated in MTT solution ...
[摘要] 通常通过3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑鎓溴化物（MTT）测定评估不同化合物的细胞毒性。该测定主要用于研究细胞系中的细胞活力（Carmichael等人，1987）。在这项研究中，该协议正在被用来确定植物根的细胞活力，用不同的应激诱导剂处理。该检测的基础是染料进入活细胞的线粒体，然后被还原成不溶性的甲，，通过直接用有机溶剂（DMSO）处理细胞来溶解甲an。颜色强度与产生的甲amount量成正比。
在本研究中，植物处理16小时，用几种植物毒性剂，然后将根在MTT溶液中温育4小时。为了溶解甲，，将根切除。用2N氢氧化钾（KOH）与DMSO一起溶解细胞壁组分，从而释放DMSO溶液中的甲granules颗粒。通过测量甲color的颜色强度来测量细胞存活率。
【背景】该协议旨在直接从根组织中确定植物细胞活力。迄今为止，MTT测定已被广泛用于哺乳动物细胞增殖和活力测定。在植物的情况下，通常使用2,3-双 - （2-甲氧基-4-硝基-5-磺苯基）-2H-四唑-5-甲酰苯胺（XTT）测定法（Kaundal等人， 2012）。成本效益的MTT分析也可以用于植物组织活力测定而不是XTT测定。该方案可用于确定不同应激诱导剂和其IC ...
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