Author:
Date:
2017-11-20
[Abstract] According to the World Health Organization (WHO), at least 50% of emerging viruses endowed with pathogenicity in humans can infect the Central Nervous System (CNS) with induction of encephalitis and other neurologic diseases (Taylor et al., 2001; Olival and Daszak, 2005). While neurological diseases are progressively documented, the underlying cellular and molecular mechanisms involved in virus infection and dissemination within the CNS are still poorly understood (Swanson and McGavern, 2015; Ludlow et al., 2016). For example, measles virus (MeV) can infect neural cells, and cause a persistent brain infections leading to lethal encephalitis from several months to years after primary infection with no available treatment (Reuter and Schneider-Schaulies, 2010; Laksono et ...
[摘要] 根据世界卫生组织(WHO)的统计,至少有50%的新发病毒具有致病性,可感染中枢神经系统(CNS),并诱发脑炎和其他神经系统疾病(Taylor et al。 >,2001; Olival和Daszak,2005)。虽然神经系统疾病逐渐被记录下来,但涉及病毒感染和在CNS内传播的潜在细胞和分子机制仍然知之甚少(Swanson and McGavern,2015; Ludlow等人,2016)。例如,麻疹病毒(MeV)可以感染神经细胞,并在原发感染后几个月至数年导致持续的脑感染,导致致死性脑炎,而没有可用的治疗(Reuter和Schneider-Schaulies,2010; Laksono等人。,2016)。器官型脑文化(OBC)是病毒学领域的一个合适的模型,以更好地理解中枢神经系统感染。实际上,它不仅可以研究中枢神经系统内嗜神经病毒的感染和传播,而且还可以作为创新性抗病毒策略或分子的筛选模型,如我们最近发表的有关融合抑制肽和HSP90伴侣蛋白活性抑制剂的研究, 17-DMAG(Welsch等人,2013; ...
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Author:
Date:
2017-11-05
[Abstract] There are two neurogenic niches in the adult mammalian brain: the subventricular zone of the lateral ventricle and the subgranular zone of the hippocampal dentate gyrus. Cells from these areas can be isolated and maintained in vitro, using two different culture systems to assess their potential regarding proliferation and differentiation in a reductionist model. While the neurosphere assay is primarily performed to directly study the proliferative and differentiation potential of cells in individual brains, the monolayer culture allows single cell analysis in a rather homogeneous cell population. Here, we describe the isolation, culturing methods and differentiation of neural precursor cells in both systems.
[摘要] 成年哺乳动物脑中有两个神经生态位:侧脑室下脑室区和海马齿状回颗粒下区。 来自这些区域的细胞可以在体外分离和维持,使用两种不同的培养系统评估它们在还原模型中的增殖和分化的潜力。 虽然神经球测定主要是为了直接研究个体脑中细胞的增殖和分化潜能,单层培养允许在相当均匀的细胞群中进行单细胞分析。 在这里,我们描述了两个系统中的神经前体细胞的分离,培养方法和分化。
【背景】在哺乳动物脑中,成人神经干细胞存在于两个主要神经生态位中,即海马齿状回(DG)的下颗粒区(SGZ)和室下区(SVZ)的侧脑室,其允许新生神经元成人的大脑。来自神经生态位的神经前体细胞可以在体外分离和培养以模拟细胞过程,尤其是增殖和分化。两种标准培养系统,贴壁单层培养(Palmer等人,1995; Ray等人,1995)和神经球测定(Reynolds和Weiss,1992和1996 )在20世纪90年代被引入,代表了在体外研究神经祖细胞生物学的有价值的工具。
根据研究问题,每个系统都有其优点和缺点,在选择其中一种或另一种培养方法之前应该仔细考虑。在贴壁单层培养中,细胞生长相当孤立,形成更均匀的培养物。单层允许直接调查和监测单细胞水平的神经前体细胞。受控条件下的形态,增殖和分化等特征可以很容易地分析和可视化。然而,与神经球培养物相比,以单层培养的细胞代表更复杂的模型,因为细胞通常以更少的通常存在于细胞壁中的细胞 ...
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