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iMark Filter absorbance microplate reader

iMark TM微孔板吸光度读数器

Company: Bio-Rad Laboratories
Catalog#: 1681135
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Glucose Production Assay in Primary Mouse Hepatocytes
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2012-11-05
[Abstract]  Hepatic glucose production is a primary determinant of fasting hyperglycemia in type 2 diabetic patients. Glucagon-cAMP-PKA pathway increases, but insulin-PI3 kinase-Akt pathway suppresses glucose production. This assay aims to evaluate the ability of isolated mouse hepatocytes to release newly synthesized glucose mainly from lactate and pyruvate as the substrates (i.e. gluconeogenesis) under basal, cAMP-, or cAMP plus insulin-treated condition. [摘要]  肝葡萄糖产生是2型糖尿病患者的空腹高血糖的主要决定因素。 胰高血糖素-cAMP-PKA通路增加,但胰岛素-PI3激酶-akt通路抑制葡萄糖产生。 该测定旨在评价分离的小鼠肝细胞主要从作为在基础,cAMP或cAMP加胰岛素治疗的条件下的底物(即,糖原异生)的乳酸盐和丙酮酸释放新合成的葡萄糖的能力。

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