| Identification of Socially-activated Neurons
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Author:
Date:
2020-09-05
[Abstract] Determining the neuronal circuitry responsible for specific behaviors is a major focus in the field of neurobiology. Activity-dependent immediate early genes (IEGs), transcribed and translated shortly after neurons discharge action potentials, have been used extensively to either identify or gain genetic access to neurons and brain regions involved in such behaviors. By using immunohistochemistry for the protein product of the IEG c-Fos combined with retrograde labeling of specific neuronal populations, precise experimental timing, and identical data acquisition and processing, we present a method to quantitatively identify specific neuronal subpopulations that were active during social encounters. We have previously used this method to show a stronger recruitment of ventral hippocampal ...
[摘要] [摘要] 确定负责特定行为的神经元回路是神经生物学领域的主要重点。在神经元释放动作电位后不久就转录和翻译的依赖活动的立即早期基因（IEG）已被广泛用于识别或获得涉及此类行为的神经元和大脑区域的遗传途径。通过对IEG c- Fos 的蛋白质产物使用免疫组织化学，结合特定神经元群体的逆向标记，精确的实验时间，相同的数据采集和处理 ng，我们提出了一种方法，可以定量地识别在社交活动中活跃的特定神经元亚群。与社交活动后投射到下丘脑外侧的神经海马神经元相比，我们以前曾使用这种方法显示出投射到内侧前额叶皮层的腹侧海马神经元更强的募集。在优化用于逆行示踪剂注射的手术后，该方法将有助于识别和定位参与许多不同行为的神经元群体。
[背景] 神经生物学领域的基本目标是确定大脑如何处理感觉输入并产生行为输出命令。在过去的二十年中，某些大脑区域已成为控制特定行为的典范实例，例如海马体用于位置记忆（Bird和Burgess，2008年）。最近的工作开始以可以推广到多种行为的方式来解析这些区域的特定功能（Aronov 等人，2017），并确定在由远程神经元连接的给定行为中多个大脑区域的作用投影（ ...
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| A Blood-retina Barrier Permeability Assay in Young Mice Using Sulfo-NHS-LC-biotin Perfusion
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Author:
Date:
2018-10-20
[Abstract] Brain and retinal vasculatures exhibit restricted vascular permeability known as blood-brain barrier and blood-retina barrier. Vascular permeability can be evaluated by perfusion of the amine reactive ester derivatives of biotin such as sulfo-NHS-LC-biotin. This protocol describes experimental procedures of sulfo-NHS-LC-biotin perfusion to evaluate retinal vascular permeability. Perfused sulfo-NHS-LC-biotin remained within vessels in wild-type postnatal day 15 (P15) retinas, confirming an intact blood-retina barrier. In contrast, sulfo-NHS-LC-biotin was occasionally detected in extravascular spaces in perfused Eogt−/− retinas suggesting a partly impaired vascular integrity in the absence of Eogt (Sawaguchi et al., 2017).
[摘要] 脑和视网膜脉管系统表现出受限的血管通透性，称为血脑屏障和血 - 视网膜屏障。 血管通透性可以通过灌注生物素的胺反应性酯衍生物如磺基-NHS-LC-生物素来评估。 该方案描述了磺基-NHS-LC-生物素灌注的实验程序，以评估视网膜血管通透性。 灌注的磺基-NHS-LC-生物素保留在野生型出生后第15天（P15）视网膜的血管内，证实了完整的血 - 视网膜屏障。 相比之下，在灌注的 Eogt - / - >视网膜中，偶尔会在血管外空间检测到磺基-NHS-LC-生物素，这表明在没有的情况下血管完整性部分受损。 Eogt >（Sawaguchi et al。>，2017）。
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| Dissection and Whole Mount Staining of Retina from Neonatal Mice
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Author:
Date:
2018-10-05
[Abstract] Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice (Sawaguchi et al., 2017) by modifying the original protocols (Powner et al., 2012; Tual-Chalot et al., 2013). This protocol can also be used for whole mount staining of adult retina.
[摘要] 在这里，我们提供了小鼠视网膜整体染色的详细方案。 该方案用于分析新生小鼠的视网膜血管生成（Sawaguchi et al。，2017），修改原始方案（Powner et al。，2012; Tual-Chalot et al。，2013）。 该方案也可用于成人视网膜的整体染色。
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