Deoxyribonuclease I

脱氧核糖核酸酶I

Company: MP Biomedicals

Catalog#: 02100575

Preparation and Purification of β-1,3-glucan-Linked Candida glabrata Cell Wall Proteases by Ion-Exchange Chromatography, Gel Filtration, and MDPF-Gelatin-Zymography Assay

Pirjo Pärnänen, Timo Sorsa, Taina Tervahartiala and Pirjo Nikula-Ijäs

Published online: 2024-03-20

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Isolation of Inner Membrane Vesicles from Escherichia coli by Using an Affinity Tag

Author:

Gang Li and Kevin D. Young,

Date:

2012-10-20

[Abstract] This protocol was developed in a project aimed to identify the inner membrane proteins localizing to cell poles in Escherichia coli (E. coli). By using a known polar protein Tar as a tag, we isolated pole-derived inner membrane vesicles by affinity capture. The specificity of the polar vesicle isolation was confirmed by mass spectrometry that identified more than one hundred proteins, most of which are known inner membrane proteins, including other known polar proteins. This protocol, or if adapted properly by choosing other affinity targets, is well suited to isolate other membrane domains of interest for identification of proteins or lipid composition. [摘要] 该方案是在旨在鉴定定位于大肠杆菌（E.coli）中的细胞极的内膜蛋白的项目中开发的。通过使用已知的极性蛋白质Tar作为标签，我们通过亲和捕获分离杆来源的内膜囊泡。极性囊泡分离的特异性通过质谱法证实，其鉴定了超过一百个蛋白质，其中大多数是已知的内膜蛋白，包括其他已知的极性蛋白质。该方案或者如果通过选择其它亲和靶适当适当地适合于分离其它感兴趣的膜结构域以鉴定蛋白质或脂质组成。

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Deoxyribonuclease I

脱氧核糖核酸酶I

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