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Author:
Date:
2012-09-20
[Abstract] Angiogenesis is involved not only in pathological conditions including cancer biology and non-neoplastic diseases, but also many biological processes including reproduction, development and repair. During angiogenesis, endothelial cells (ECs) undergo activation after binding of angiogenic factors to their receptors, release of proteases to dissolve the basement membrane, migration towards an angiogenic signal, proliferation, and an increase in cell number for new blood vessel formation. Finally, reorganization of ECs forms the three-dimensional vasculature. HUVEC tube-formation assay is one of the simple, but well-established in vitro angiogenesis assays based on the ability of ECs to form three-dimensional capillary-like tubular structures, when cultured on a gel of growth ...
[摘要] 血管生成不仅涉及包括癌症生物学和非肿瘤疾病的病理状况，而且涉及许多生物过程，包括繁殖，发育和修复。 在血管生成期间，内皮细胞（EC）在血管生成因子与其受体结合之后经历活化，释放蛋白酶以溶解基底膜，向血管生成信号迁移，增殖和用于新血管形成的细胞数目的增加。 最后，EC的重组形成三维脉管系统。 当在生长凝胶上培养时，HUVEC管形成测定是基于EC形成三维毛细管样管状结构的能力的简单的，但是良好建立的体外血管生成测定法之一 因子减少基底膜提取物。 在测定期间，ECs分化，定向迁移以排列，分支并形成管状多边形网络的血管。
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