Potassium Dihydrogen Phosphate
Company: Wako Pure Chemical Industries
Catalog#: 169-04245
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EAE Induction by Passive Transfer of MOG-specific CD4+ T Cells
Author:
Date:
2017-07-05
[Abstract]  Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS), which is a chronic inflammatory disease of the central nervous system (CNS). It is characterized by focal demyelination and inflammatory responses mediated by myelin-specific autoreactive CD4+ T cells. Using a passive transfer model of EAE in mice, we have demonstrated that regional specific neural signals by sensory-sympathetic communications create gateways for immune cells at specific ...

Heavy Metal Stress Assay of Caenorhabditis elegans
Author:
Date:
2017-06-05
[Abstract]  Organisms have developed many protective systems to reduce the toxicity from heavy metals. The nematode Caenorhabditis elegans has been widely used to determine the protective mechanisms against heavy metals. Responses against heavy metals can be monitored by expression of reporter genes, while sensitivity can be determined by quantifying growth or survival rate following exposure to heavy metals.

Phos-tag Immunoblot Analysis for Detecting IRF5 Phosphorylation
Author:
Date:
2017-05-20
[Abstract]  While the activation of the transcription factor interferon regulatory factor 5 (IRF5) is critical for the induction of innate immune responses, it also contributes to the pathogenesis of the autoimmune disease systemic lupus erythematosus (SLE). IRF5 phosphorylation is a hallmark of its activation in the Toll-like receptor (TLR) pathway, where active IRF5 induces type I interferon and proinflammatory cytokine genes. By using the phosphate-binding molecule Phos-tag, without either radioisotopes ...

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