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Author:
Date:
2020-12-05
[Abstract] During immune responses, B cells home to lymph nodes (LN), where they encounter antigens. Homing starts with capture and L-selectin-dependent rolling on the activated endothelium of high endothelial venules (HEV). After recognition of chemokines presented on HEV, activation of B cell integrins occurs mediating firm arrest. Subsequently, B cells crawl to the spot of extravasation to enter the LN. Extravasation can be visualized and quantified in vivo by intravital microscopy (IVM) of the inguinal LN. Here, we describe an established protocol that permits detailed in vivo analysis of B cell recruitment to LN under sterile inflammatory conditions. We describe data acquisition, exportation, quantification, and statistical analysis using specialized software. IVM of LN is a powerful technique ...
[摘要] [摘要]在免疫反应期间，B细胞归巢于淋巴结（LN ），在那里它们遇到抗原。归巢始于在高内皮小静脉（HEV）的活化内皮细胞上进行捕获和L-选择蛋白依赖性滚动。在识别出存在于HEV上的趋化因子后，B细胞整合素的激活发生了介导的牢固停滞。随后，B细胞爬到外渗点进入LN。外渗可通过腹股沟淋巴结的活体显微镜检查（IVM）进行体内可视化和定量。在这里，我们描述了一个已建立的协议，该协议允许在无菌炎症条件下对LN的B细胞募集的详细体内分析。我们描述了数据采集，出口，定量，并使用专门的软件进行统计分析。LN的IVM是一种强大的技术，可以更好地了解体内炎症过程中B细胞的迁移行为。
[背景]次级LN在战术上定位为通过淋巴系统排出抗原，因此在人体不同部位的免疫反应中起着基本作用。这意味着原位免疫细胞的募集，抗原的遭遇以及免疫反应的增强（Tan and Watanabe，2010; Tavares et ...
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