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Sodium bicarbonate

碳酸氢钠

Company: Sigma-Aldrich
Catalog#: S5761
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Qualitative in vivo Bioluminescence Imaging
Author:
Date:
2018-09-20
[Abstract]  Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in exploring tumor growth and metastatic spread in real-time. This technique ensures the optimal use of laboratory animal resources, particularly the ethical principle of reduction in animal use, given its non-invasive nature, ensuring that ongoing biological processes can be studied over time in the same animal, without the need to euthanize groups of mice at specific time points. In this protocol, the luciferase imaging technique was developed to study the effect of co-inoculating pericytes (contractile, αSMA+ mesenchymal ... [摘要]  生物发光成像(BLI)技术是一种在体内实验室动物上进行分子成像的先进方法。 这种强大的技术广泛应用于研究各种生物过程,是实时探索肿瘤生长和转移扩散的理想工具。 该技术确保实验室动物资源的最佳利用,特别是减少动物使用的伦理原则,考虑到其非侵入性,确保可以在同一动物中随时间研究正在进行的生物过程,而无需安乐死 小鼠在特定的时间点。 在该方案中,开发了荧光素酶成像技术以研究共同接种周细胞(收缩性,αSMA + 间充质干细胞样细胞,位于微血管内的细胞)对生长和转移性扩散的影响。 卵巢癌使用侵袭性卵巢癌细胞系-OVCAR-5-作为例子。

【背景】生物发光成像(BLI)的原理是基于相对简单的生化过程的发光特性,即,荧光素酶介导的分子底物荧光素氧化产生光。在癌症研究中,BLI是一种流行的工具(Contag et ...

Soluble and Solid Iron Reduction Assays with Desulfitobacterium hafniense
Author:
Date:
2018-09-05
[Abstract]  There is a pressing need to develop sustainable and efficient methods to protect and stabilize iron objects. To develop a conservation-restoration method for corroded iron objects, this bio-protocol presents the steps to investigate reductive dissolution of ferric iron and biogenic production of stabilizing ferrous iron minerals in the strict anaerobe Desulfitobacterium hafniense (strains TCE1 and LBE). We investigated iron reduction using three different Fe(III) sources: Fe(III)-citrate (a soluble phase), akaganeite (solid iron phase), and corroded coupons. This protocol describes a method that combines spectrophotometric quantification of the complex Fe(II)-Ferrozine® with mineral characterization by scanning electron microscopy and Raman spectroscopy. These three ... [摘要]  迫切需要开发可持续和有效的方法来保护和稳定铁制物体。为了开发腐蚀铁物体的保护 - 恢复方法,该生物方案提出了研究严格厌氧菌[Desulfitobacterium hafniense (菌株TCE1)中三价铁的还原溶解和稳定亚铁矿物质的生物产生的步骤。和LBE)。我们使用三种不同的Fe(III)来源研究了铁还原:Fe(III) - 柠檬酸盐(可溶相),akaganeite(固体铁相)和腐蚀的试样。该协议描述了一种方法,该方法结合了复杂的Fe(II)-Ferrozine ®的分光光度定量,通过扫描电子显微镜和拉曼光谱进行矿物表征。这三种方法可以评估三价铁的还原溶解和生物矿物质生产,作为开发一种创新的可持续方法来稳定腐蚀铁的有希望的替代方法。

【背景】自铁器时代以来,铁已被用于生产日常用具。因此,考古学上的铁试验是过去极其重要的证据,应予以保留。然而,由于其反应性,铁容易被腐蚀并且考古铁物体可能被完全损坏。埋藏时,铁制品会根据埋葬地点的环境条件形成复杂的腐蚀层。挖掘后,条件发生变化,腐蚀层变得不稳定。为避免完全破坏,考古铁制物需要快速稳定处理。目前,可用的稳定化处理不能提供长期保护并且具有实质性缺点,例如毒性,低效率和大量废物的产生(Scott和Eggert,2009; Rimmer 等人, 2012)。因此,有必要开发新技术来稳定考古铁器。

越来越多地考虑利用微生物代谢来开发更有效,可持续和环保的保护 ...

Fluorescent Labeling of Rat-tail Collagen for 3D Fluorescence Imaging
Author:
Date:
2018-07-05
[Abstract]  Rat tail collagen solutions have been used as polymerizable in vitro three-dimensional (3D) extracellular matrix (ECM) gels for single and collective cell migration assays as well as spheroid formation. These 3D hydrogels are a relatively inexpensive, simple to use model system that can mimic the in vivo physical characteristics of numerous tissues within the body, namely the skin. While confocal imaging techniques such as fluorescence reflection and two-photon microscopy are able to visualize collagen fibrils during 3D imaging without fluorescence, other imaging modalities require direct conjugation of fluorescent dyes to collagen. Here we detail how to generate 3D collagen gels labeled with a fluorescent dye. Furthermore, we go through the steps required to ... [摘要]  大鼠尾胶原溶液已被用作可聚合的体外三维(3D)细胞外基质(ECM)凝胶,用于单一和集体细胞迁移测定以及球状体形成。 这些3D水凝胶是相对便宜,易于使用的模型系统,其可以模拟体内许多组织(即皮肤)的体内物理特征。 虽然诸如荧光反射和双光子显微镜的共焦成像技术能够在没有荧光的3D成像期间可视化胶原原纤维,但是其他成像模式需要荧光染料直接缀合到胶原。 在这里,我们详细介绍了如何生成用荧光染料标记的3D胶原凝胶。 此外,我们还经历了可重复生成适用于活细胞3D成像技术的明亮胶原水凝胶所需的步骤。

【背景】自20世纪50年代以来,Paul Weiss和Beatrice Garber最初观察到增加血浆浓度(纤维蛋白)对间充质细胞形态的影响(Weiss和Garber,1952),开始研究细胞迁移和细胞与周围微环境的相互作用。在随后的几十年中,生物化学家开始深入研究从鼠尾胶原中纯化提取物,并开始将其用作高度可聚合的3D基质(Fitch et al。,1955; Gross et al。,1955; Chandrakasan et al。,1976)。直到20世纪90年代,3D矩阵才真正对细胞生物学界有用,尤其是研究细胞迁移(Friedl et ...

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