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Author:
Date:
2012-05-05
[Abstract] Antigen recognition and activation of T cell receptor (TCR) triggers transient calcium release from intracellular compartments and subsequent sustained calcium influx through cell surface Icrac channels. Sustained elevation of the cytoplasmic calcium level activates many calcium-dependent enzymes and transcription factors, which are essential for T cell activation and function. This protocol uses non-ratiometric dyes, in combination with flow cytometry, to monitor TCR-triggered calcium changes over time, and is a simple assay to examine the existence of T cell calcium mobilization defects in transgenic mice.
[摘要] 抗原识别和T细胞受体（TCR）的激活触发从细胞内区室瞬时钙释放和随后通过细胞表面Icrac通道的持续钙流入。 细胞质钙水平的持续升高激活许多钙依赖性酶和转录因子，其对于T细胞活化和功能是必需的。 该协议使用非比率染料，结合流式细胞术，监测TCR触发的钙随时间的变化，是一个简单的测定，检查转基因小鼠的T细胞钙动员缺陷的存在。
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