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PierceTM NeutrAvidinTM Agarose

Pierce TM NeutrAvidin TM琼脂糖

Company: Thermo Fisher Scientific
Catalog#: 29200
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Biotinylation of Cell Surface Proteins
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Date:
2012-05-05
[Abstract]  Membrane proteins are major sensors of extracellular stimuli and initiators of intracellular signal transduction, and their abundance on the cell surface in particular is often dynamically regulated even when there are no significant changes of their total abundance in a cell. This protocol is designed to biochemically label and separate membrane proteins on the plasma membrane from those in the intracellular compartments. In conjunction with co-immunoprecipitation and western blot analysis, functional analysis of dynamic interaction of membrane proteins with other membrane proteins or intracellular adaptor and effector proteins can be achieved. [摘要]  膜蛋白是胞外刺激和细胞内信号转导的启动子的主要传感器,并且它们在细胞表面上的丰度特别是经常动态调节,即使当它们在细胞中的总丰度没有显着变化时。 该协议设计为生物化学标记和分离质膜上的膜蛋白与细胞内区室中的膜蛋白。 结合共免疫沉淀和蛋白质印迹分析,可以实现膜蛋白与其他膜蛋白或细胞内接头和效应子蛋白的动态相互作用的功能分析。

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