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Author:
Date:
2018-03-20
[Abstract] Corn, or maize, is a major cereal crop and model monocot. Two of its benefits are ease of pollination and tractable genetics. For a comparison of development between maize and the dicot model, Arabidopsis, see Lunde and Hake, 2005. For an example of how this protocol might be used for a double mutant analysis see Lunde and Hake, 2009. Here, we provide a protocol for easy DNA extraction without phenol or chloroform. Therefore, this protocol is suitable for use in schools or laboratories that lack fume hoods.
[摘要] 玉米或玉米是主要的谷类作物和模式单子叶植物。 它的两个好处是易于授粉和易处理的遗传。 为了比较玉米和双子叶植物模型之间的发育，拟南芥参见Lunde和Hake，2005。关于该方案如何用于双突变体分析的例子参见Lunde和Hake，2009。 在这里，我们提供了一个简单的DNA提取方案，无需苯酚或氯仿。 因此，该协议适用于缺乏通风橱的学校或实验室。
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