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Author:
Date:
2014-08-05
[Abstract] It has recently been reported that tomato fruit chromoplasts can synthesize ATP de novo using an ATP synthase complex harboring an atypical γ-subunit which is also present in a variety of plant species. However many aspects related with the biochemical processes underlying this process remain largely unknown. Here we describe detailed protocols for the isolation of tomato fruit chromoplasts and the determination of ATP levels (end-point measurements) and ATP synthesis rates (kinetic measurements) in these organelles using bioluminescent luciferin/luciferase based assays.
[摘要] 最近报道了番茄果实色素可以使用含有非典型γ亚基的ATP合酶复合物来合成ATP，所述亚基也存在于多种植物物种中。 然而，与这个过程基础的生化过程相关的许多方面仍然很大程度上未知。 在这里我们描述了隔离番茄果实色质体和ATP水平（终点测量）和ATP合成率（动力学测量）在这些细胞器中使用基于生物发光荧光素/荧光素酶的测定的确定详细协议。
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