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Sigma 1-16K

Sigma 1-16K

Company: Sigma Laborzentrifugen
Catalog#: 1-16K
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Measurement of Chlorophyll a and Carotenoids Concentration in Cyanobacteria
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2015-05-05
[Abstract]  This is a protocol for precise measurement of chlorophyll a and total carotenoid concentrations in cyanobacteria cells. Cellular chlorophyll concentration is one of the central physiological parameters, routinely followed in many research areas ranging from stress physiology to biotechnology. Carotenoids concentration is often related to cellular stress level; combined pigments assessment provides useful insight into cellular physiological state. The current protocol was established to minimize time and equipment requirements for the routine pigments analysis. It is important to note that this protocol is suitable only for cyanobacteria containing chlorophyll a, and is not designed for species containing other chlorophyll molecules. [摘要]  这是用于精确测量蓝细菌细胞中叶绿素a和总类胡萝卜素浓度的方案。 细胞叶绿素浓度是中心生理参数之一,在从应激生理学到生物技术的许多研究领域中常规地遵循。 类胡萝卜素浓度通常与细胞应激水平相关; 组合颜料评估提供了有用的洞察细胞生理状态。 建立了当前方案以使对常规色素分析的时间和设备要求最小化。 重要的是注意,该方案仅适用于含有叶绿素a的蓝细菌,并且不被设计用于含有其他叶绿素分子的物种。

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