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ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

Company: Sigma-Aldrich
Catalog#: E1270
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Tumor Cell Invasion Assay
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2012-02-05
[Abstract]  Cell invasion assays have been used to study the interactions between tumor cells and the extracellular matrix (ECM), which not only provides a structural scaffold for the cell, but also contains various biological factors for the survival and growth of the cell. ECM gel contains the basic components of the ECM that provides a structural support for the cell to grow and move. Cells can secrete enzymes that degrade certain components of the ECM to move towards chemoattractants, or to simply establish niches for growth. Metastatic tumor cells often show more invasiveness to the ECM gel due to their higher motility and/or enzymatic activity for degrading ECM components. This protocol describes a tumor cell invasion assay to study the interactions between tumor cells and the ECM. [摘要]  细胞侵袭测定已经用于研究肿瘤细胞和细胞外基质(ECM)之间的相互作用,其不仅为细胞提供结构支架,而且还包含用于细胞存活和生长的各种生物因子。 ECM凝胶含有ECM的基本组分,为细胞生长和移动提供结构支持。 细胞可分泌降解ECM的某些组分以趋向化学引诱物的酶,或简单地建立用于生长的壁龛。 转移性肿瘤细胞通常对ECM凝胶显示更多的侵袭性,这是由于它们对于降解ECM组分的较高的运动性和/或酶活性。 该协议描述了肿瘤细胞侵袭实验,以研究肿瘤细胞和ECM之间的相互作用。

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